机构：[1]Department of ICU, Shanghai Xuhui District Central Hospital, Shanghai, China [2]Department of Galactophore, The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong Province, China 大德路总院乳腺科大德路总院乳腺科广东省中医院[3]Health Care Center, Shanghai University of Medicine & Health Sciences, Shanghai, China [4]Department of Respiratory Medicine, Shanghai Xuhui District Central Hospital, Shanghai, China
The aim of the study was to explore the effect of lipoxin A4 (LXA4) on lung injury in sepsis rats through the p38/mitogen-activated protein kinase (MAPK) signaling pathway. Sprague-Dawley rats were used for the study. The rat model of sepsis-induced acute lung injury was established via cecal ligation (Sepsis group, n=20). LXA4 (0.1 mg/kg) was injected at 6 h after modeling (Treatment group, n=20), and a The Control group (n=20) was also set up. The 7-day survival rate was 100% in The Control group, and LXA4 raised the survival rate of rats in the Sepsis group from 40% to 60% (P<0.01). Alveolar fluid clearance (AFC) significantly declined and the wet/dry weight (W/D) ratio of lung tissues rose remarkably in the Sepsis group compared with those in the Control group, while LXA4 restored AFC and reduced the W/D ratio of lung tissues (P<0.05), suggesting that LXA4 treatment reduces lung fluids and partially enhances AFC, thus lowering the W/D ratio of lung. The total cell count, polymorphonuclear neutrophils (PMN) percentage and concentration of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 in bronchoalveolar lavage fluid (BALF) were obviously increased in the Sepsis group compared with those in the Control group, while they were markedly decreased in the Treatment group (P<0.05). The activity of myeloperoxidase (MPO) in lung tissue homogenate was evidently higher in the Sepsis group than that in The Control group, while it was notably lower in the Treatment group than that in the Sepsis group after LXA4 treatment (P<0.05). Moreover, it was observed microscopically that the morphology of lung tissues was intact in the Control group. Finally, the results of Western blotting manifested that the p-p38/ MAPK protein expression was remarkably increased in the Sepsis group, indicating the activation of the p38/MAPK pathway, while it was remarkably decreased in the Treatment group, indicating the inhibited activity of the pathway (P<0.05). LXA4 has an anti-inflammatory effect on sepsis rats with lung injury, and such effect is related to the p38/MAPK signaling pathway.Copyright 2020 Biolife Sas. www.biolifesas.org.
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外文
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出版当年[2019]版：
大类|4 区医学
小类|4 区内分泌学与代谢4 区免疫学4 区医学：研究与实验4 区生理学
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第一作者机构：[1]Department of ICU, Shanghai Xuhui District Central Hospital, Shanghai, China
推荐引用方式(GB/T 7714)：
Hu X H,Situ H L,Chen J P,et al.Lipoxin A4 alleviates lung injury in sepsis rats through p38/MAPK signaling pathway.[J].Journal of biological regulators and homeostatic agents.2020,34(3):807-814.doi:10.23812/20-108-A-20.
APA：
Hu X H,Situ H L,Chen J P&Yu R H.(2020).Lipoxin A4 alleviates lung injury in sepsis rats through p38/MAPK signaling pathway..Journal of biological regulators and homeostatic agents,34,(3)
MLA：
Hu X H,et al."Lipoxin A4 alleviates lung injury in sepsis rats through p38/MAPK signaling pathway.".Journal of biological regulators and homeostatic agents 34..3(2020):807-814
