机构：[1]Traditional Chinese Pharmacological Laboratory, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.[2]School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.[3]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.[4]Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, Southern Medical University, Guangzhou, China.[5]Nanfang Hospital of Southern Medical University, Guangzhou, China.
Danggui Niantong Granules (DGNTG) are a valid and reliable traditional herbal formula, commonly used in clinical practice to treat rheumatoid arthritis (RA). However, the mechanism of its effect on RA remains unclear.An investigation of the therapeutic effects of DGNTG on RA.Twenty-four male Sprague-Dawley (SD) rats were divided into four groups: control, model, DGNTG (2.16 g/kg, gavage), methotrexate (MTX) (1.35 mg/kg, gavage) for 28 days. The morphology of synovial and ankle tissues was observed by haematoxylin-eosin staining. The responses of mitochondrial apoptosis were assessed by qPCR, Western blotting and immunohistochemical staining. Rat faeces were analysed by 16S rRNA sequencing.Our results showed that DGNTG treatment reduced AI scores (7.83 ± 0.37 vs. 4.67 ± 0.47, p < 0.01) and paw volumes (7.63 ± 0.17 vs. 6.13 ± 0.11, p < 0.01) compared with the model group. DGNTG also increased the expression of Bax (0.34 ± 0.03 vs. 0.73 ± 0.03, p < 0.01), cytochrome c (CYTC) (0.24 ± 0.02 vs. 0.64 ± 0.01, p < 0.01) and cleaved caspase-9 (0.24 ± 0.04 vs. 0.83 ± 0.08, p < 0.01), and decreased bcl-2 (1.70 ± 0.11 vs. 0.60 ± 0.09, p < 0.01) expression. DGNTG treatment regulated the structure of gut microbiota.DGNTG ameliorated RA by promoting mitochondrial apoptosis, which may be associated with regulating gut microbiota structure. DGNTG can be used as a supplement and alternative drug for the treatment of RA; its ability to prevent RA deserves further study.
基金：
This work was supported by the Natural Science Foundation of
Guangdong Province [grant number 2022A1515011681], Natural
Science Foundation of China (NSFC) [grant number 8167151349]
and Natural Science Foundation of China (NSFC) [grant number
8177150993].
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|3 区医学
小类|2 区医学实验技术3 区植物科学3 区药学
最新[2025]版：
大类|3 区医学
小类|2 区医学实验技术2 区药学2 区植物科学
第一作者：
第一作者机构：[1]Traditional Chinese Pharmacological Laboratory, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
共同第一作者：
通讯作者：
通讯机构：[3]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.[4]Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, Southern Medical University, Guangzhou, China.[5]Nanfang Hospital of Southern Medical University, Guangzhou, China.[*1]Nanfang Hospital of Southern Medical University, Guangzhou, China[*2]Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, Southern Medical University, Guangzhou, China
推荐引用方式(GB/T 7714)：
Lu Qi-Jin,Li Jia-Yu,Lin Hong-Xin,et al.Danggui Niantong granules ameliorate rheumatoid arthritis by regulating intestinal flora and promoting mitochondrial apoptosis[J].Pharmaceutical biology.2022,60(1):1606-1615.doi:10.1080/13880209.2022.2107018.
APA：
Lu Qi-Jin,Li Jia-Yu,Lin Hong-Xin,Cai Yi-Si,Liu Chang-Shun...&Yuan Li-Xia.(2022).Danggui Niantong granules ameliorate rheumatoid arthritis by regulating intestinal flora and promoting mitochondrial apoptosis.Pharmaceutical biology,60,(1)
MLA：
Lu Qi-Jin,et al."Danggui Niantong granules ameliorate rheumatoid arthritis by regulating intestinal flora and promoting mitochondrial apoptosis".Pharmaceutical biology 60..1(2022):1606-1615
