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CCL3 Promotes Proliferation of Colorectal Cancer Related with TRAF6/NF-κB Molecular Pathway

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机构： [1]Department of Colorectal Surgery, General Hospital of Ningxia Medical University, Yinchuan 750003, China. [2]College of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, China. [3]Surgical Department, General Hospital of Ningxia Medical University, Yinchuan 750003, China. [4]Operating Room, General Hospital of Ningxia Medical University, Yinchuan 750003, China. [5]Surgical Department, Lingwu Traditional Chinese Medicine Hospital, Yinchuan 750499, China. [6]Department of Colorectal and Anal Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. [7]Department of General Surgery, Chengdu Second People's Hospital, Chengdu 610021, China. [8]Institute of Clinical Laboratory Medicine, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, School of Medical Technology, Dongguan 523000, China.

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Chemokine C-C motif chemokine ligand 3 (CCL3) plays an important role in the invasion and metastasis of malignant tumors. For developing new therapeutic targets and antitumor drugs, the effect of chemokine CCL3 and the related cytokine network on colorectal cancer should be investigated. This study used cell, tissue, and animal experiments to prove that CCL3 is highly expressed in colorectal cancer and confirmed that CCL3 can promote the proliferation of cancer cells, and its expression is closely related to TRAF6/NF-κB molecular pathway. In addition, protein chip technology was used to examine colorectal cancer tissue samples and identify the key factors of chemokine CCL3 and the toll-like receptors/nuclear factor-κB (TLR/NF-κB) pathway in cancer and metastatic lymph nodes. Furthermore, the lentiviral vector technology was employed for transfection to construct interference and overexpression cell lines. The experimental results reveal the mechanism of CCL3 and TNF receptor-associated factor 6 (TRAF6)/NF-κB pathway-related factors and their effects on the proliferation of colon cancer cells. Finally, the expression and significance of CCL3 in colorectal cancer tissues and its correlation with clinical pathology were studied by immunohistochemistry. Also, the results confirmed that CCL3 and C-C motif chemokine receptor 5 (CCR5) were expressed in adjacent tissues, colorectal cancer tissues, and metastatic cancer. The expression level was correlated with the clinical stage and nerve invasion. The expression of chemokine CCL3 and receptor CCR5 was positively correlated with the expression of TRAF6 and NF-κB and could promote the proliferation, invasion, and migration of colorectal cancer cells through TRAF6 and NF-κB.Copyright © 2022 Xiaoqiang Ma et al.

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出版当年[2021]版：

大类 | 4 区医学

小类 | 4 区核医学

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第一作者：

第一作者机构： [1]Department of Colorectal Surgery, General Hospital of Ningxia Medical University, Yinchuan 750003, China. [2]College of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, China.

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通讯作者：

通讯机构： [1]Department of Colorectal Surgery, General Hospital of Ningxia Medical University, Yinchuan 750003, China. [2]College of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, China.

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