机构：[1]Research Center for Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.[2]The Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, People's Republic of China.[3]Natural Products Research Laboratories, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina 27599, United States.[4]Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.[5]Chinese Medicine Research and Development Center, China Medical University and Hospital, Taichung, Taiwan.
Bioassay-guided fractionation was conducted on an EtOAc-soluble extract of the whole plants of Scutellaria barbata, monitored by inhibition of Epstein-Barr virus (EBV) lytic replication. Twenty-six neo-clerodane diterpenoids were isolated, of which 13 are new (1-13, scutolides A-L) and 13 previously known (14-26). The structures of 1-13 were elucidated by analysis of their NMR and MS spectroscopic data. Furthermore, the configurations of the new compounds 1 and 11 were confirmed by single-crystal X-ray diffraction. All of the isolated compounds were evaluated for inhibitory effects against EBV lytic replication. Eleven compounds (3, 4, 6, 11, 12, 15, 16, 17, 20, 22, and 24) exhibited moderate to potent inhibition, with EC50 values from 3.2 to 23.6 μM and selective index (SI) values from 2.1 to 109.2. More specifically, the new compound 4 showed the most potent activity, with EC50 and SI values of 3.2 μM and 46.1, respectively, while compound 24 (EC50 = 16.4 μM) exhibited the highest SI of 109.2. This study is the first to report that neo-clerodane diterpenoids demonstrate significant inhibition against EBV lytic replication.
基金：
National High-Tech
R&D Program of China (863 Program) (2012AA020307), the
Guangdong Innovative Research Team Program (No.
2009010058), and the National Natural Science Foundation
of China (Nos. 81173470, 81271805, 81171575, 81371793).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2014]版：
大类|3 区生物
小类|2 区药物化学2 区植物科学3 区药学
最新[2025]版：
大类|2 区生物学
小类|2 区药物化学2 区药学2 区植物科学
第一作者：
第一作者机构：[1]Research Center for Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
共同第一作者：
通讯作者：
通讯机构：[1]Research Center for Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.[3]Natural Products Research Laboratories, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina 27599, United States.[5]Chinese Medicine Research and Development Center, China Medical University and Hospital, Taichung, Taiwan.
推荐引用方式(GB/T 7714)：
Wu Taizong,Wang Qian,Jiang Cheng,et al.Neo-clerodane diterpenoids from Scutellaria barbata with activity against Epstein-Barr virus lytic replication[J].Journal of natural products.2015,78(3):500-9.doi:10.1021/np500988m.
APA：
Wu Taizong,Wang Qian,Jiang Cheng,Morris-Natschke Susan L,Cui Hui...&Gu Qiong.(2015).Neo-clerodane diterpenoids from Scutellaria barbata with activity against Epstein-Barr virus lytic replication.Journal of natural products,78,(3)
MLA：
Wu Taizong,et al."Neo-clerodane diterpenoids from Scutellaria barbata with activity against Epstein-Barr virus lytic replication".Journal of natural products 78..3(2015):500-9
