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Single-cell RNA-seq and chromatin accessibility profiling decipher the heterogeneity of mouse γδ T cells

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收录情况： ◇ 统计源期刊 ◇ CSCD-C ◇ 卓越：领军期刊

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机构： [1]Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment, Zhuhai Institute of Translational Medicine, Zhuhai People’s Hospital Affiliated with Jinan University, Jinan University, Zhuhai 519000, China [2]The Biomedical Translational Research Institute, Faculty of Medical Science, Jinan University, Guangzhou 510632, China [3]The First Affiliated Hospital, Faculty of Medical Science, Jinan University, Guangzhou 510000, China [4]Emergency Department, The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou 510000, China [5]OE Biotech Co., Ltd., Shanghai 201114, China [6]Institute of Molecular Rhythm and Metabolism, Guangzhou University of Chinese Medicine, Guangzhou 510700, China [7]Zhuhai People’s Hospital (Zhuhai Hospital Affiliated with Jinan University), Jinan University, Zhuhai 519000, China

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The distinct characteristics of γδ T cells determine their vital roles in the formation of local immune responses and contribute to tissue homeostasis. However, the heterogeneity of γδ T cells across tissues remains unclear. By combining transcriptional and chromatin analyses with a truly unbiased fashion, we constructed a single-cell transcriptome and chromatin accessibility landscape of mouse γδ T cells in the lymph, spleen, and thymus. We also revealed the heterogeneity of γδ T1 and γδ T17 cells across these tissues and inferred their potential regulatory mechanisms. In the thymus, we reconstructed the developmental trajectory and gained further insights into the signature genes from the mature stage, intermediate stage, and immature stage of γδ T cells on the basis of single-cell RNA sequencing and single-cell assay for transposase-accessible chromatin sequencing data. Notably, a novel Gzma+ γδ T cell subset was identified with immature properties and only localized to the thymus. Finally, NR1D1, a circadian transcription factor (TF), was validated as a key and negative regulator of γδ T17 cell differentiation by performing a combined analysis of TF motif enrichment, regulon enrichment, and Nr1d1 knockout mice. In summary, our data represent a comprehensive mapping on the transcriptome and chromatin accessibility dynamics of mouse γδ T cells, providing a valuable resource and reference for future studies on γδ T cells.Copyright © 2021 Science China Press. Published by Elsevier B.V. All rights reserved.

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出版当年[2021]版：

大类 | 1 区

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小类 | 1 区综合性期刊

第一作者：

第一作者机构： [1]Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment, Zhuhai Institute of Translational Medicine, Zhuhai People’s Hospital Affiliated with Jinan University, Jinan University, Zhuhai 519000, China [2]The Biomedical Translational Research Institute, Faculty of Medical Science, Jinan University, Guangzhou 510632, China

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通讯机构： [1]Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment, Zhuhai Institute of Translational Medicine, Zhuhai People’s Hospital Affiliated with Jinan University, Jinan University, Zhuhai 519000, China [2]The Biomedical Translational Research Institute, Faculty of Medical Science, Jinan University, Guangzhou 510632, China

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