机构：[1]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.[2]Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.[3]Department of Biochemistry and Molecular Biology, Guangdong Provincial Key Laboratory of Single Cell Technology and Application, School of Basic Medical Science, Southern Medical University, Guangzhou 510515, China.[4]The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China.[5]School of Medicine, Tsinghua University, Beijing 100084, China.[6]Department of Rheumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.[7]College of Basic Medicine and Life Sciences, Hainan Medical University, Haikou 571199, China.
Radioactive substances have been used in various aspects in daily life. However, high-energy radiation could cause environmental problems, which would damage the human body. Circular RNA (CircRNA) has great potential in the minimization of ionizing radiation damage. To find a potential diagnostic and therapeutic target for reducing the damage of ionizing radiation, we selected circRNA cleavage and polyadenylation specificity factor subunit 1 (circ-CPSF1) based on its up-regulated expression after X-ray radiation and explored its effect on response to ionizing radiation using Caenorhabditis elegans (C. elegans). Circ-CPSF1 was screened out and its up-regulated expression was verified. The measurement of lifespan and germ cell apoptosis showed that circ-CPSF1 RNAi treatment extended lifespan and reduced apoptotic germ cells. ROS levels were significantly reduced after the interference of circ-CPSF1 in C. elegans with radiation. Mitochondrial membrane potential assay showed that the suppression of circ-CPSF1 could alleviate mitochondrial damage after radiation. Relative genes expression showed the involvement of circ-CPSF1 in radiation mediated DNA damage response pathways and apoptosis pathways. In conclusion, circ-CPSF1 exerts deleterious effects on lifespan, eggs production and germ cell apoptosis of C. elegans through oxidative stress, the DNA damage response (DDR) pathway, and the core apoptotic pathway after ionizing radiation, indicating the potential of circ-CPSF1 to be an important therapeutic target of radiation damage.
基金：
This research was funded by National Natural Science Foundation of China, 81872557; the Key Research and Development Foundation of Hainan Province, ZDYF2021SHFZ223; Guangzhou Basic and Applied Basic Research Project, 202201011800; Guangdong Basic and Applied Basic Research Foundation, 2019A15151101590; Science Foundation of Guangdong Provincial Administration Chinese Medicine, grant number, 20201221.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类|2 区生物学
小类|3 区生化与分子生物学
最新[2025]版：
大类|2 区生物学
小类|3 区生化与分子生物学
第一作者：
第一作者机构：[1]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.[2]Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
