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DIAPH3 condensates formed by liquid-liquid phase separation act as a regulatory hub for stress-induced actin cytoskeleton remodeling

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收录情况： ◇ SCIE

作者：
Zhang Ke[1] * ; Huang Miaodan[1] ; Li Ang[1] ; Wen Jing[1] ; Yan Lingli[1] ; Li Yunhao[1] ; Guo Liman[2] ; Senthil Kumaran Satyanarayanan[1] ; Zhou Yangyang[1] ; Chen Guobing[3] ; Liu Yong[4] ; Zhang Xiaofei[2] ; Yao Xiaoli[5] ; Qin Dajiang[6] ; Su Huanxing[1] ;
机构： [1]State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China [2]Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China [3]Institute of Geriatric Immunology, School of Medicine, Jinan University, Guangzhou, China [4]Laboratory of Neuroscience in Health and Disease Institute, Guangzhou First People’s Hospital School of Medicine, South China University of Technology, Guangzhou, China [5]Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China [6]Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
出处：
DOI： 10.1016/j.celrep.2022.111986
ISSN：

摘要：
Membraneless condensates, such as stress granules (SGs) and processing bodies (P-bodies), have attracted wide attention due to their unique feature of rapid response to stress without first requiring nuclear feedback. In this study, we identify diaphanous-related formin 3 (DIAPH3), an actin nucleator, as a scaffold protein to initiate liquid-liquid phase separation (LLPS) and form abundant cytosolic phase-separated DIAPH3 granules (D-granules) in mammalian cells such as HeLa, HEK293, and fibroblasts under various stress conditions. Neither mRNAs nor known stress-associated condensate markers, such as G3BP1, G3BP2, and TIA1 for SGs and DCP1A for P-bodies, are detected in D-granules. Using overexpression and knockout of DIAPH3, pharmacological interventions, and optogenetics, we further demonstrate that stress-induced D-granules spatially sequester DIAPH3 within the condensation to inhibit the assembly of actin filaments in filopodia. This study reveals that D-granules formed by LLPS act as a regulatory hub for actin cytoskeletal remodeling in response to stress.Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

基金：
This work was supported by the funding from Macau Science and Technology Development Fund (0061/ 2021/A2) and the Science and Technology Development Fund, Macau SAR (SKL-QRCM(UM)-2023-2025).
语种：
外文
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中科院(CAS)分区：
出版当年[2022]版：
大类 | 1 区 生物学
小类 | 2 区 细胞生物学
最新[2025]版：
大类 | 1 区 生物学
小类 | 2 区 细胞生物学
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出版当年[2021]版：
Q1 CELL BIOLOGY
最新[2023]版：
Q1 CELL BIOLOGY

影响因子： 最新[2023版] 最新五年平均 出版当年[2021版] 出版当年五年平均 出版前一年[2020版] 出版后一年[2022版]

第一作者：
第一作者机构： [1]State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Ke,Huang Miaodan,Li Ang,et al.DIAPH3 condensates formed by liquid-liquid phase separation act as a regulatory hub for stress-induced actin cytoskeleton remodeling[J].CELL REPORTS.2023,42(1):doi:10.1016/j.celrep.2022.111986.
APA：
Zhang Ke,Huang Miaodan,Li Ang,Wen Jing,Yan Lingli...&Su Huanxing.(2023).DIAPH3 condensates formed by liquid-liquid phase separation act as a regulatory hub for stress-induced actin cytoskeleton remodeling.CELL REPORTS,42,(1)
MLA：
Zhang Ke,et al."DIAPH3 condensates formed by liquid-liquid phase separation act as a regulatory hub for stress-induced actin cytoskeleton remodeling".CELL REPORTS 42..1(2023)

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