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Hydroxychloroquine protects against autoimmune premature ovarian insufficiency by modulating the Treg/Th17 cell ratio in BALB/c mice

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机构： [1]Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China. [2]The First Affiliated Hospital of Shenzhen University, Reproductive Medicine Centre, Shenzhen Second People's Hospital, Shenzhen, China. [3]Department of Obstetrics and Gynecology, Guangdong Provincial People's Hospital, Guangzhou, China. [4]School of Materials Science and Engineering, South China University of Technology, Guangzhou, China. [5]Department of Medicine, Shenzhen University, Shenzhen, China. [6]Department of Gynecology and Obstetrics, Graduate College of Guangxi University of Traditional Chinese Medicine, Nanning, China. [7]Shantou University Medical College, Shantou, China. [8]Guangdong Medical University, Zhanjiang, China.

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The role of hydroxychloroquine (HCQ) in premature ovarian insufficiency (POI) remains unclear. The purpose of this study was to evaluate the effect of HCQ on ovarian function in mice with POI and to clarify its potential mechanisms.POI was induced in mice by injection with zona pellucida 3 peptide (pZP3), and HCQ was administered intragastrically. Stages of the estrous cycle were determined using vaginal cytology. The ovarian structure was observed under a microscope after hematoxylin-eosin staining. The levels of serum hormones and anti-ZP antibody (aZPAb) were measured using enzyme-linked immunosorbent assay (ELISA). The expression levels of CD4, CD45, and ZP2, ZP3 were determined using immunofluorescence and immunohistochemistry, respectively. The T regulatory (Treg)/ T helper 17 (Th17) cell ratio was analyzed using flow cytometry analysis. Western blotting was performed to assess the expression levels of proteins, transcription factors and cytokines.Administration of HCQ to mice with POI greatly restored their estrus cycle. In the treatment group compared to the POI group, estradiol (E2 ) levels were higher, and follicle stimulating hormone (FSH) levels were lower. In addition, following pZP3, HCQ treatment increased ZP2 and ZP3 expression. Additionally, by inhibiting the activation of the TLR7 signaling pathway, HCQ attenuated the infiltration of inflammatory cells and prevented the activated naive CD4+ T cells from developing into Th17 cells.Our findings showed that HCQ effectively restored ovarian function by altering the Treg/Th17 cell ratio in mice with POI, indicating that HCQ maybe a promising therapeutic method for patients with POI.© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

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出版当年[2022]版：

大类 | 3 区医学

小类 | 3 区生殖生物学 4 区免疫学

最新[2025]版：

大类 | 4 区医学

小类 | 4 区免疫学 4 区生殖生物学

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出版当年[2021]版：

Q2 REPRODUCTIVE BIOLOGY Q3 IMMUNOLOGY

最新[2023]版：

Q3 IMMUNOLOGY Q3 REPRODUCTIVE BIOLOGY

影响因子： 2.5 最新[2023版] 3.2 最新五年平均 3.777 出版当年[2021版] 3.99 出版当年五年平均 3.886 出版前一年[2020版] 3.6 出版后一年[2022版]

第一作者：

第一作者机构： [1]Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China. [2]The First Affiliated Hospital of Shenzhen University, Reproductive Medicine Centre, Shenzhen Second People's Hospital, Shenzhen, China.

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通讯作者：

通讯机构： [1]Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China. [2]The First Affiliated Hospital of Shenzhen University, Reproductive Medicine Centre, Shenzhen Second People's Hospital, Shenzhen, China. [*1]Department ofObstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China [*2]The First Affiliated Hospital of Shenzhen University, ReproductiveMedicine Centre, Shenzhen Second People’s Hospital, Shenzhen 518035, China

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