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Fetal bovine serum, an important factor affecting the reproducibility of cell experiments

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机构： [1]Department of Laboratory, Shenzhen Samii International Medical Center (Shenzhen Fourth People’s Hospital), Shenzhen 518118, People’s Republic of China. [2]Institute of Biomedical Engineering, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University [3]The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, Guangdong, People’s Republic of China. [4]Department of Clinical Laboratory, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen 518033, People’s Republic of China.

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Fetal bovine serum (FBS) is a natural medium used in cell cultures containing the large amount of nutrients necessary for cell growth and is often used for in vitro cultures of animal cells. Although FBS plays a vital role in cell cultures, there are small molecules contained within FBS that remain unidentified, and their effects on cultured cells is poorly understood. Here, we report that different brands of FBS have varying influences on the background expression of IL-8, not TNFα and IL1β in epithelial cells. The endogenous small molecules in FBS and ERK pathways may contribute to these effects. In addition, FBS form the IL-8 stimulation and IL-8 non-responsive groups have different metabolome profiles. Overall, our study suggests that metabolites in FBS should be included in the quantitative considerations when conducting cell experiments, especially immune-related experiments, to improve the repeatability of experimental results in scientific papers; IL-8 could thus be an important factor in selecting FBS.© 2023. The Author(s).

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出版当年[2022]版：

大类 | 3 区综合性期刊

小类 | 3 区综合性期刊

最新[2025]版：

大类 | 3 区综合性期刊

小类 | 3 区综合性期刊

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出版当年[2021]版：

Q2 MULTIDISCIPLINARY SCIENCES

最新[2023]版：

Q1 MULTIDISCIPLINARY SCIENCES

影响因子： 3.8 最新[2023版] 4.3 最新五年平均 4.997 出版当年[2021版] 5.516 出版当年五年平均 4.38 出版前一年[2020版] 4.6 出版后一年[2022版]

第一作者：

第一作者机构： [1]Department of Laboratory, Shenzhen Samii International Medical Center (Shenzhen Fourth People’s Hospital), Shenzhen 518118, People’s Republic of China.

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通讯作者：

通讯机构： [2]Institute of Biomedical Engineering, Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University [3]The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, Guangdong, People’s Republic of China.

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Fetal bovine serum, an important factor affecting the reproducibility of cell experiments