Objective: This study aims to estimate the prevalence of anti-mitochondrial antibody subtype M2 (AMA-M2) and assess its consistency with AMA in a general population.Methods: A total of 8954 volunteers were included to screen AMA-M2 using enzyme-linked immunosorbent assay. Sera with AMA-M2 >50 RU/mL were further tested for AMA using an indirect immunofluorescence assay.Results: The population frequency of AMA-M2 positivity was 9.67%, of which 48.04% were males and 51.96% were females. The AMA-M2 positivity in males had a peak and valley value of 7.81% and 16.88% in those aged 40 to 49 and =70 years, respectively, whereas it showed a balanced age distribution in females. Transferrin and immunoglobulin M were the risk factors for AMA-M2 positivity and exercise was the only protective factor. Of 155 cases with AMA-M2 >50 RU/mL, 25 cases were AMA-positive, with a female-to-male ratio of 5.25:1. Only 2 people, with very high AMA-M2 of 760 and >800 RU/mL, met the diagnostic criteria of primary biliary cholangitis (PBC), making the prevalence of PBC 223.36 per million in southern China.Conclusion: We found that AMA-M2 has a low coincidence rate with AMA in the general population. A new decision-making point for AMA-M2 is needed to improve consistency with AMA and diagnostic accuracy.
基金：
Science and Technology Projects in Guangzhou [202102021139]; Science and technology research project of traditional Chinese Medicine of Guangdong Provincial Hospital of Chinese Medicine [YN2019QL06]; GuangDong Basic and Applied Basic Research Foundation [SZ2021ZZ24]; Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases [2018B030322012]
第一作者机构：[1]Guangzhou Univ Chinese Med, Affiliated Hosp 2, Dept Lab Med, Guangzhou, Peoples R China[2]Guangzhou Univ Chinese Med, Affiliated Hosp 2, State Key Lab Dampness Syndrome Chinese Med, Guangzhou, Peoples R China
通讯作者：
通讯机构：[1]Guangzhou Univ Chinese Med, Affiliated Hosp 2, Dept Lab Med, Guangzhou, Peoples R China[2]Guangzhou Univ Chinese Med, Affiliated Hosp 2, State Key Lab Dampness Syndrome Chinese Med, Guangzhou, Peoples R China[4]Guangzhou Univ Chinese Med, Affiliated Hosp 2, Guangdong Prov Key Lab Chinese Med Prevent & Treat, Guangzhou, Peoples R China
推荐引用方式(GB/T 7714)：
Liang En-yu,Liu Miao,Ke Pei-feng,et al.A population-based characterization study of \nanti-mitochondrial M2 antibodies and its consistency with anti-mitochondrial antibodies[J].LABORATORY MEDICINE.2023,54(6):618-625.doi:10.1093/labmed/lmad018.
APA：
Liang, En-yu,Liu, Miao,Ke, Pei-feng,Han, Guang,Zhang, Cheng...&Huang, Xian-zhang.(2023).A population-based characterization study of \nanti-mitochondrial M2 antibodies and its consistency with anti-mitochondrial antibodies.LABORATORY MEDICINE,54,(6)
MLA：
Liang, En-yu,et al."A population-based characterization study of \nanti-mitochondrial M2 antibodies and its consistency with anti-mitochondrial antibodies".LABORATORY MEDICINE 54..6(2023):618-625
