机构：[1]School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, P. R. China.[2]Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028 Jiangsu Province, PR China.[3]School of Environmental Science, Nanjing XiaoZhuang University, Nanjing 211171, PR China.[4]School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen, Guangdong 518060, P. R. China.
Quantitative measurement of microRNAs (miRNAs) is extremely important in plenty of biomedical applications especially cancer diagnosis but remains a great challenge. In this work, we developed a logic gate recognition biosensing platform based on the "trinity" molecular recognition mode for quantifying miRNAs with a detection limit of 4.48 aM, along with a linear range from 0.1 nM to 10 aM under optimal experimental conditions. In order to obtain excellent detection performance, we adopted a Zn(TCPP) photocatalytic electron transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization signal amplification strategy. The light-induced PET-RAFT has developed green applications of free radical polymerization in the field of biosensors. This is the first report on the preparation of signal amplification biosensors using PET-RAFT for tumor marker detection. With the outstanding detection performance, we can apply the sensor system to the early screening of lung cancer patients.
基金：
This work was supported by the National Natural Science
Foundation of China (nos. 21974068, 21890742, 9195401,
and 21890740).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类|2 区材料科学
小类|2 区纳米科技2 区材料科学：综合
最新[2025]版：
大类|2 区材料科学
小类|2 区材料科学：综合2 区纳米科技
第一作者：
第一作者机构：[1]School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu 210094, P. R. China.[2]Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028 Jiangsu Province, PR China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Sun Haobo,Liu Jingliang,Kong Jinming,et al.Ultrasensitive miRNA-21 Biosensor Based on Zn(TCPP) PET-RAFT Polymerization Signal Amplification and Multiple Logic Gate Molecular Recognition[J].ACS applied materials & interfaces.2023,15(14):17716-17725.doi:10.1021/acsami.3c02428.
APA：
Sun Haobo,Liu Jingliang,Kong Jinming,Zhang Jian&Zhang Xueji.(2023).Ultrasensitive miRNA-21 Biosensor Based on Zn(TCPP) PET-RAFT Polymerization Signal Amplification and Multiple Logic Gate Molecular Recognition.ACS applied materials & interfaces,15,(14)
MLA：
Sun Haobo,et al."Ultrasensitive miRNA-21 Biosensor Based on Zn(TCPP) PET-RAFT Polymerization Signal Amplification and Multiple Logic Gate Molecular Recognition".ACS applied materials & interfaces 15..14(2023):17716-17725
材料科学