Temporal oscillations in intestinal nutrient processing and absorption are coordinated by the local clock, which leads to the hypothesis that intestine clock has major impact on shaping peripheral rhythms via diurnal nutritional signals. Here we investigate the role of intestine clock in controlling liver rhythmicity and metabolism.Transcriptomic analysis, metabolomics, metabolic assays, histology, qPCR, and immunoblotting were performed with Bmal1-iKO (intestine-specific knockout of Bmal1), Rev-erbα-iKO and control mice.Bmal1-iKO causes a large-scale reprogramming of the rhythmic transcriptome of mouse liver with a limited effect on its clock. In the absence of intestinal Bmal1, liver clock is resistant to the entrainment by inverted feeding and high-fat diet. Importantly, Bmal1-iKO remodels diurnal hepatic metabolism by shifting to gluconeogenesis from lipogenesis in the dark phase, leading to elevated glucose production (hyperglycemia) and insulin insensitivity. Conversely, Rev-erbα-iKO causes a diversion to lipogenesis from gluconeogenesis in the light phase, resulting in enhanced lipogenesis and an increased susceptibility to alcoholic liver injury. These temporal diversions are attributed to a disruption of hepatic SREBP-1c rhythmicity, which is maintained via gut-derived polyunsaturated fatty acids produced by intestinal FADS1/2 under the control of local clock.Our findings establish a pivotal role of intestine clock in dictating liver rhythmicity and diurnal metabolism, and suggest targeting intestinal rhythms as a new avenue for improving metabolic health.Our findings establish the centrality of the intestine clock amongst peripheral tissue clocks, and associate liver-related pathologies with malfunction of intestine clock. Clock modifiers in the intestine are shown to modulate liver metabolism with improved metabolic parameters. The knowledge will help the clinicians improve the diagnosis and treatment of metabolic diseases by incorporating intestinal circadian factors.Copyright © 2023 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.