The role of Semen Ziziphus jujube saponins in sedative and hypnosishas attracted much attention.The study aimed to investigate its possible UV damage protection andanti-aging effects. Total saponins (SZR I) and purified saponins (SZRII) were analyzed and compared by infrared spectroscopy and high-performanceliquid chromatography (HPLC). The protective effects of SZR I, SZRII, and their three monomers on HaCaT cells damaged by UV were studied,and their anti-aging activities were observed by Caenorhabditiselegans with paraquat-induced oxidative stress. Theresults showed that SZR I and SZR II differ in chemical compositionbut both have the same three monomers. The cell survival rate treatedwith SZR I and SZR II at a concentration of 400 & mu;g/mL increasedby 34.45 and 88.98%, respectively, indicating that they could promotethe proliferation of UVB-damaged HaCaT cells. Jujuboside A, JujubosideB, and spinosin from the saponins exhibited similar effects on UVB-damagedHaCaT cells. SZR I and SZR II had little effect on reproductive performancebut could delay the senescence caused by heat and oxidative stressof the C. elegans model. These resultsprovide useful data that Semen Z. jujube saponin is a potential natural product with UV damage protectionand anti-aging characteristics.