个人中心| | 帮助
高级检索
当前位置： 首页 > 详情页

Hemolysin from Aeromonas hydrophila enhances the host's serum enzyme activity and regulates transcriptional responses in the spleen of Cyprinus rubrofuscus

| 认领 | 导出 | |

文献详情

资源类型：
Pubmed体系：
作者：
Li Mei[1,2,3,*2] * ; Li Dan[2] ; Li Fenglan[1] ; Liu Wenli[1] ; Wang Shuang[3] ; Wu Gongqing[3] ; Wu Guofeng[1] ; Tan Guiliang[1] ; Zheng Ziyi[1] ; Li Lin[1] ; Pan Ziqiang[1] ; Liu Yiyao[2,4,*1] ;
机构： [1]School of Material Science and Food Engineering, University of Electronic Science and Technology of China Zhongshan Institute, Zhongshan 528402, China [2]School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610072, China [3]College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China [4]TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan, China
出处：
DOI： 10.1016/j.ecoenv.2023.115375
ISSN：

关键词： Differentially expressed genes Aeromonas hydrophila Koi carp (Cyprinus rubrofuscus) Hemolysin Transcriptome

摘要：
Aeromonas hydrophila is a conditional pathogen impacting public hygiene and safety. Hemolysin is a virulence factor of Aeromonas hydrophila that causes erythrocyte hemolysis, yet its transcriptional response to Cyprinus rubrofuscus remains unknown. Our investigation confirmed the hemolysis of hemolysin from A. hydrophila. Serum enzyme activity was evaluated weekly after C. rubrofuscus were immunized with hemolysin Ahh1. The results showed that the hemolysin enhances the serum superoxide dismutase (SOD), lysozyme (LZM), and catalase (CAT) activity, which reached a maximum on day 14. To elucidate the molecular interaction between hemolysin from A. hydrophila and the host, we performed transcriptome sequencing on the spleen of C. rubrofuscus 14 days post hemolysin infection. The total number of clean reads was 41.37 Gb, resulting in 79,832 unigenes with an N50 length of 1863 bp. There were 1982 significantly differentially expressed genes (DEGs), including 1083 upregulated genes and 899 downregulated genes. Transcript levels of the genes, such as LA6BL, CD2, and NLRC5, were significantly downregulated, while those of IL11, IL1R2, and IL8 were dramatically upregulated. The DEGs were mainly enriched in the immune disease, viral protein interaction with cytokine and cytokine receptor, and toll-like receptor pathways, suggesting that hemolysin stimulation can activate the transcriptional responses. RT-qPCR experiments results of seven genes, IL-8, STAT2, CTSK, PRF1, CXCL9, TLR5, and SACS, showed that their expression was highly concordant with RNA-seq data. We clarified for the first time the key genes and signaling pathways response to hemolysin from A. hydrophila, which offers strategies for treating and preventing diseases.Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类 | 2 区 环境科学与生态学
小类 | 1 区 毒理学 2 区 环境科学
最新[2025]版：
大类 | 2 区 环境科学与生态学
小类 | 1 区 毒理学 2 区 环境科学
第一作者：
第一作者机构： [1]School of Material Science and Food Engineering, University of Electronic Science and Technology of China Zhongshan Institute, Zhongshan 528402, China [2]School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610072, China [3]College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China [*2]School of Material Science and Food Engineering, University of Electronic Science and Technology of China Zhongshan Institute, Zhongshan 528402, China
通讯作者：
通讯机构： [1]School of Material Science and Food Engineering, University of Electronic Science and Technology of China Zhongshan Institute, Zhongshan 528402, China [2]School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610072, China [3]College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China [4]TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan, China [*1]School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610072, China. [*2]School of Material Science and Food Engineering, University of Electronic Science and Technology of China Zhongshan Institute, Zhongshan 528402, China
推荐引用方式(GB/T 7714)：
Li Mei,Li Dan,Li Fenglan,et al.Hemolysin from Aeromonas hydrophila enhances the host's serum enzyme activity and regulates transcriptional responses in the spleen of Cyprinus rubrofuscus[J].Ecotoxicology and environmental safety.2023,263:115375.doi:10.1016/j.ecoenv.2023.115375.
APA：
Li Mei,Li Dan,Li Fenglan,Liu Wenli,Wang Shuang...&Liu Yiyao.(2023).Hemolysin from Aeromonas hydrophila enhances the host's serum enzyme activity and regulates transcriptional responses in the spleen of Cyprinus rubrofuscus.Ecotoxicology and environmental safety,263,
MLA：
Li Mei,et al."Hemolysin from Aeromonas hydrophila enhances the host's serum enzyme activity and regulates transcriptional responses in the spleen of Cyprinus rubrofuscus".Ecotoxicology and environmental safety 263.(2023):115375

相关文献

[1]变应性鼻炎合并哮喘差异性表达基因筛选研究 [2]Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes. [3]Comprehensive Transcriptome Sequencing Analysis of Hirudinaria manillensis in Different Growth Periods. [4]Comparative transcriptome analysis of Gentiana macrophylla seeds after ferrous-priming and hydro-priming [5]湿热质人群差异表达基因的筛选及其功能研究 [6]Transcriptomic reveals the ferroptosis features of host response in a mouse model of Zika virus infection [7]A Novel miRNA-mRNA Axis Involves in Regulating Transcriptional Disorders in Pancreatic Adenocarcinoma. [8]慢性胃炎脾气虚证与脾胃湿热证的差异表达基因比较 [9]Exploring the pathogenesis of colorectal carcinoma complicated with hepatocellular carcinoma via microarray data analysis [10]Identification of key pathways and biomarkers in sorafenib-resistant hepatocellular carcinoma using bioinformatics analysis.

资源点击量：2027 今日访问量：0 总访问量：659 更新日期：2024-07-01 建议使用谷歌、火狐浏览器 常见问题

版权所有©2020 广东省中医院 技术支持：重庆聚合科技有限公司 地址：广州市越秀区大德路111号