机构：[1]Department of Neurosurgery & Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde Foshan), Foshan, China.[2]Transformation Engineering Research Center of Chronic Disease Diagnosis and Treatment, Department of Physiology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, Guizhou, China.[3]Center for Clinical Medical Education, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.[4]Department of Oncology, Guizhou Cancer Hospital, Guiyang, Guizhou, China.[5]Guangdong Provincial Key Laboratory of Molecular Oncologic Pathology, Southern Medical University, Guangzhou, China.[6]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, China.
Translocation of 14-3-3 protein epsilon (14-3-3ε) was found to be involved in Triptolide (Tp)-induced inhibition of colorectal cancer (CRC) cell proliferation. However, the form of cell death induced by 14-3-3ε translocation and mechanisms underlying this effect remain unclear. This study employed label-free LC-MS/MS to identify 14-3-3ε-associated proteins in CRC cells treated with or without Tp. Our results confirmed that heterogeneous nuclear ribonucleoproteins C1/C2 (hnRNP C) were exported out of the nucleus by 14-3-3ε and degraded by ubiquitination. The nucleo-cytoplasmic shuttling of 14-3-3ε carrying hnRNP C mediated Tp-induced proliferation inhibition, cell cycle arrest and autophagic processes. These findings have broad implications for our understanding of 14-3-3ε function, provide an explanation for the mechanism of nucleo-cytoplasmic shuttling of hnRNP C and provide new insights into the complex regulation of autophagy.
基金：
The work was supported by the Guizhou Education Department Youth Science and Technology Talents Growth Project [KY[2018]173]; National Natural Science Foundation of China [81472315]; Natural Science Foundation of Guangdong Province [2022A1515012552].
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类|3 区医学
小类|4 区肿瘤学
最新[2025]版：
大类|2 区医学
小类|3 区肿瘤学
第一作者：
第一作者机构：[1]Department of Neurosurgery & Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde Foshan), Foshan, China.[2]Transformation Engineering Research Center of Chronic Disease Diagnosis and Treatment, Department of Physiology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, Guizhou, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Guo Manlan,He Minyi,Zhang Yi,et al.Nucleo-cytoplasmic shuttling of 14-3-3 epsilon carrying hnRNP C promotes autophagy[J].Cancer biology & therapy.2023,24(1):2246203.doi:10.1080/15384047.2023.2246203.
APA：
Guo Manlan,He Minyi,Zhang Yi,Liu Weiwen,Qi Min...&Liu Yawei.(2023).Nucleo-cytoplasmic shuttling of 14-3-3 epsilon carrying hnRNP C promotes autophagy.Cancer biology & therapy,24,(1)
MLA：
Guo Manlan,et al."Nucleo-cytoplasmic shuttling of 14-3-3 epsilon carrying hnRNP C promotes autophagy".Cancer biology & therapy 24..1(2023):2246203
