机构:[1]Guangzhou Univ Chinese Med, Sch Clin Med 2, Guangzhou 510405, Peoples R China广东省中医院[2]Guangzhou Univ Chinese Med, Guangzhou Higher Educ Mega Ctr, Sch Basic Med Sci, Lab TCM Syndrome Essence & Objectificat, 232 East Waihuan Rd, Guangzhou 510006, Peoples R China[3]Army Med Univ, Southwest Hosp, Dept Rheumatol & Immunol, Chongqing 400038, Peoples R China[4]NanoFCM Inc, Xiamen 361006, Peoples R China
Hyperlipidemia, a common metabolic disease, is a risk factor for cardiovascular diseases, Poria cocos (PC) and Alismatis rhizoma (AR) serve as a potential treatment. A systematic approach based on transcriptome sequencing analysis and bioinformatics methods was developed to explore the synergistic effects of PC-AR and identify major compounds and potential targets. The phenotypic characteristics results indicated that the high dose (4.54 g/kg) of PC-AR reduced total cholesterol (TC), elevated high-density lipoprotein cholesterol (HDL-C) levels, and improved hepatocyte morphology, as assessed via hematoxylin and eosin (H&E) staining. Transcriptomic profiling processing results combined with GO enrichment analysis to identify the overlapping genes were associated with inflammatory responses. The cytokine-cytokine receptor interaction pathway was found as a potential key pathway using geneset enrichment analysis. Core enrichment targets were selected according to the PC-AR's fold change versus the model. Real-time quantitative PCR analysis validated that PC-AR significantly downregulated the expression of Cxcl10, Ccl2, Ccl4, Cd40 and Il-1 beta mRNA (P < 0.05). Molecular docking analysis revealed the significant compounds of PC-AR and the potential binding patterns of the critical compounds and targets. This study provides further evidence that the therapeutic effects of PC-AR on hyperlipidemia in rats through the regulation of inflammation-related targets.
基金:
Natural Science Foundation of Guangdong Province [2020A1515010725]; Key Areas of Research and Development Plan of Guangdong Province [2020B1111100001]
第一作者机构:[1]Guangzhou Univ Chinese Med, Sch Clin Med 2, Guangzhou 510405, Peoples R China
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通讯作者:
推荐引用方式(GB/T 7714):
Zhou Xiaowen,Luo Jingbiao,Lin Shuxian,et al.Efficacy of Poria cocos and Alismatis rhizoma against diet-induced hyperlipidemia in rats based on transcriptome sequencing analysis[J].SCIENTIFIC REPORTS.2023,13(1):doi:10.1038/s41598-023-43954-6.
APA:
Zhou, Xiaowen,Luo, Jingbiao,Lin, Shuxian,Wang, Yaxin,Yan, Zhenqian...&Li, Xiantao.(2023).Efficacy of Poria cocos and Alismatis rhizoma against diet-induced hyperlipidemia in rats based on transcriptome sequencing analysis.SCIENTIFIC REPORTS,13,(1)
MLA:
Zhou, Xiaowen,et al."Efficacy of Poria cocos and Alismatis rhizoma against diet-induced hyperlipidemia in rats based on transcriptome sequencing analysis".SCIENTIFIC REPORTS 13..1(2023)