机构:[1]Guangzhou Univ Chinese Med, Clin Coll 2, Guangzhou, Peoples R China广东省中医院[2]Guangdong Prov Hosp Chinese Med, Guangzhou, Peoples R China广东省中医院[3]Acad Chinese Med Sci, Res Team Bone & Joint Degenerat Injury Guangdong P, Guangzhou, Peoples R China[4]Guangzhou Univ Chinese Med, Clin Med Coll 5, Guangzhou, Peoples R China[5]Guangdong Second Chinese Med Hosp, Guangdong Prov Enginering Technol Res Inst Tradit, Guangzhou, Peoples R China
Background and objective With the development of global population aging, comorbidity (>= 2 diseases) is a common health problem among elderly people. Osteoarthritis (OA) and osteoporosis (OP) are common in elderly individuals. There is a lack of drug therapy for OA and OP comorbidities. The purpose of this study was to explore the efficacy and mechanism of Longbie capsule (LBJN), which contains various plant herbs, in treating OA and OP comorbidities (OA + OP) in rats using metabolomics techniques.Methods We created an OA + OP rat model through bilateral oophorectomy combined with meniscus instability surgery. Thirty SD rats were randomly divided into five groups (six in each group), namely, the sham group, OA group, OA + OP group, LBJN low-dose group (0.625 g/kg, OA + OP+LB-L group) and LBJN high-dose group (1.25 g/kg, OA + OP+LB-H group). After 8 weeks of intervention, we used micro-CT to detect bone microstructure status, ELISA to measure bone metabolism indicators, and UPLC-MS technology for metabolomics analysis. Finally, the screened differentially expressed metabolites were subjected to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and functional enrichment analysis.Results The micro-CT results showed that LBJN significantly improved the bone mineral density (BMD) and bone quality of subchondral bone in OA + OP rats, and LBJN regulated the expression of bone alkaline phosphatase (BALP), osteoprotegerin (OPG), and tartrate-resistant acid phosphatase (TRACP) in serum to maintain bone metabolism balance. Metabolomics analysis showed that the metabolic trajectory of OA + OP rats after intervention in the OA + OP+LB-H group showed significant changes, and 107 potential biomarkers could be identified. Among them, 50 metabolites were upregulated (such as zeranol) and 57 were downregulated (such as vanillactic acid). The KEGG functional enrichment results indicated that the differentially expressed metabolites are mainly involved in amino acid metabolism, lipid metabolism, and carbohydrate metabolism. The KEGG pathway enrichment results indicated that LBJN may exert therapeutic effects on OA + OP rats by regulating the cAMP signaling pathway, and the FoxO signaling pathway.Conclusion LBJN can maintain bone metabolism balance by regulating serum lipid metabolism, amino acid metabolism, carbohydrate metabolism, and estrogen, thereby reducing bone loss in subchondral bone, which may be a potential mechanism through which LBJN treats OA + OP.
基金:
National Natural Science Foundation of China (Nos. 82305263, 82004386, 81873314, and 81974574), the National Key Research and Development Program (No. 2021YFC1712804), Guangdong Basic and Applied Basic Research Foundation (Nos. 2022A1515010385, 2022A1515011700, 2022A1515220131, and 2023A1515012626), the Project of Administration of Traditional Chinese Medicine of Guangdong Province (Nos. 20241125 and 20225016), the Science and Technology Program of Guangzhou (No. 2023A03J0238), the Research Fund for Bajian Talents of Guangdong Provincial Hospital of Chinese Medicine (No. BJ2022KY01), and the Science and Technology Research Project of Guangdong Provincial Hospital of Chinese Medicine (Nos. YN2019ML08 and YN2020MS16).
第一作者机构:[1]Guangzhou Univ Chinese Med, Clin Coll 2, Guangzhou, Peoples R China[2]Guangdong Prov Hosp Chinese Med, Guangzhou, Peoples R China[3]Acad Chinese Med Sci, Res Team Bone & Joint Degenerat Injury Guangdong P, Guangzhou, Peoples R China
共同第一作者:
通讯作者:
通讯机构:[1]Guangzhou Univ Chinese Med, Clin Coll 2, Guangzhou, Peoples R China[2]Guangdong Prov Hosp Chinese Med, Guangzhou, Peoples R China[3]Acad Chinese Med Sci, Res Team Bone & Joint Degenerat Injury Guangdong P, Guangzhou, Peoples R China[4]Guangzhou Univ Chinese Med, Clin Med Coll 5, Guangzhou, Peoples R China[5]Guangdong Second Chinese Med Hosp, Guangdong Prov Enginering Technol Res Inst Tradit, Guangzhou, Peoples R China
推荐引用方式(GB/T 7714):
Liang Guihong,Zhao Jinlong,Zhao Di,et al.Longbie capsules reduce bone loss in the subchondral bone of rats with comorbid osteoporosis and osteoarthritis by regulating metabolite alterations[J].FRONTIERS IN MEDICINE.2023,10:doi:10.3389/fmed.2023.1256238.
APA:
Liang, Guihong,Zhao, Jinlong,Zhao, Di,Dou, Yaoxing,Huang, Hetao...&Liu, Jun.(2023).Longbie capsules reduce bone loss in the subchondral bone of rats with comorbid osteoporosis and osteoarthritis by regulating metabolite alterations.FRONTIERS IN MEDICINE,10,
MLA:
Liang, Guihong,et al."Longbie capsules reduce bone loss in the subchondral bone of rats with comorbid osteoporosis and osteoarthritis by regulating metabolite alterations".FRONTIERS IN MEDICINE 10.(2023)