机构：[1]National TB Control Program, National TB Reference Laboratory, Islamabad, Pakistan.[2]Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.[3]Zhongjing Research and Industrialization Institute of Chinese Medicine, Zhongguancun Scientific Park, Nanyang, Henan, China.[4]Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Pakistan.[5]School of Medicine, Department of Immunology and Microbiology, Shanghai Jiao Tong University, Shanghai, China.[6]National Tuberculosis Clinical Lab of China, Beijing Chest Hospital, Capital Medical University, Beijing, China.[7]School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.[8]Peng Cheng Laboratory, Shenzhen, Guangdong, China.[9]Faculty of Medical Sciences, University College London, London, United Kingdom.
An accurate diagnosis of drug resistance in clinical isolates is an important step for better treatment outcomes. The current study observed a higher discordance rate of rifampicin resistance on Mycobacteria Growth Indicator Tube (MGIT) drug susceptibility testing (DST) than Lowenstein-Jenson (LJ) DST when compared with the rpoB sequencing. We detected a few novel mutations and their combination in rifampicin resistance isolates that were missed by MGIT DST and may be useful for the better management of tuberculosis (TB) treatment outcomes. Few novel deletions in clinical isolates necessitate the importance of rpoB sequencing in large data sets in geographic-specific locations, especially high-burden countries. We explored the discordance rate on MGIT and LJ, which is important for the clinical management of rifampicin resistance to avoid the mistreatment of drug-resistant TB. Furthermore, MGIT-sensitive isolates may be subjected to molecular methods of diagnosis for further confirmation and treatment options.
基金：
Quaid-e-Azam University Ethical Committee
(QAU-20BRC), Islamabad, Pakistan.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类|1 区生物学
小类|1 区微生物学
最新[2025]版：
大类|2 区生物学
小类|3 区微生物学
第一作者：
第一作者机构：[1]National TB Control Program, National TB Reference Laboratory, Islamabad, Pakistan.[2]Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
通讯作者：
通讯机构：[3]Zhongjing Research and Industrialization Institute of Chinese Medicine, Zhongguancun Scientific Park, Nanyang, Henan, China.[7]School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.[8]Peng Cheng Laboratory, Shenzhen, Guangdong, China.
推荐引用方式(GB/T 7714)：
Qadir Mehmood,Faryal Rani,Khan Muhammad Tahir,et al.Phenotype versus genotype discordant rifampicin susceptibility testing in tuberculosis: implications for a diagnostic accuracy[J].Microbiology spectrum.2023,e0163123.doi:10.1128/spectrum.01631-23.
APA：
Qadir Mehmood,Faryal Rani,Khan Muhammad Tahir,Khan Sajjad Ahmed,Zhang Shulin...&McHugh Timothy D.(2023).Phenotype versus genotype discordant rifampicin susceptibility testing in tuberculosis: implications for a diagnostic accuracy.Microbiology spectrum,,
MLA：
Qadir Mehmood,et al."Phenotype versus genotype discordant rifampicin susceptibility testing in tuberculosis: implications for a diagnostic accuracy".Microbiology spectrum .(2023):e0163123
生物学