机构：[1]Department of Clinical Laboratory, Xinhua Hospital, Shanghai Jiaotong University of Medicine School, Shanghai, 200092, China[2]Department of Pathology and Laboratory Medicine, Oregon Health and Science University, Portland, OR, 97239, USA[3]Hematology/Flow Cytometry lab, Department of Pathology, University of California Irvine Medical Center, Orange, CA, 92868, USA[4]Casey Eye Institution, Oregon Health and Science University, Portland, OR, 97239, USA[5]Department of Laboratory Medicine, Guangdong Provincial Hospital of Chinese Medicine, Zhuhai, 519015, China大德路总院珠海院区检验科大德路总院检验科广东省中医院[6]Faculty of Medical Laboratory Science, College of Health Science and Technology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China[7]Institute of Artificial Intelligence Medicine, Shanghai Academy of Experimental Medicine, Shanghai, 200070, China
To investigate the relationship between immune dynamic and graft-versus-host-disease (GVHD) risk, 111 initial diagnostic acute myeloid leukemia patients were reviewed. The flow cytometry data of 12 major lymphocyte subsets in bone marrow (BM) from 60 transplant patients at four different time points were analyzed. Additionally, 90 immune subsets in peripheral blood (PB) of 11 post-transplantation on day 100 were reviewed. Our results demonstrated that transplant patients had longer OS compared to non-transplant patients (P < 0.001). Among transplant patients, those who developed GVHD showed longer OS than those without GVHD (P < 0.05). URD donors and CMV-negative status donors were associated with improved OS in transplant patients (P < 0.05). Importantly, we observed a decreased Th/Tc ratio in BM at initial diagnostic in patients with GVHD compared to those without GVHD (P = 0.034). Receiver operating characteristic analysis indicated that a low Th/Tc ratio predicted an increased risk of GVHD with a sensitivity of 44.44% and specificity of 87.50%. Moreover, an increased T/NK ratio in BM of post-induction chemotherapy was found to be associated with GVHD, with a sensitivity of 75.76% and specificity of 65.22%. Additionally, we observed a decreased percentage of NK1 (CD56-CD16(+)NK) in PB on day 100 post-transplantation in the GVHD group (P < 0.05). These three indicators exhibit promising potential as specific and useful biomarkers for predicting GVHD. These findings provide valuable insights for the early identification and management of GVHD risk, thereby facilitating the possibility of improving patient outcomes.
基金：
Shanghai Youth Medical Talent Training Program for Clinical Laboratory (Shanghai Medicine and Health Development Foundation) [HYWJ201605]
第一作者机构：[1]Department of Clinical Laboratory, Xinhua Hospital, Shanghai Jiaotong University of Medicine School, Shanghai, 200092, China
通讯作者：
通讯机构：[1]Department of Clinical Laboratory, Xinhua Hospital, Shanghai Jiaotong University of Medicine School, Shanghai, 200092, China[2]Department of Pathology and Laboratory Medicine, Oregon Health and Science University, Portland, OR, 97239, USA[6]Faculty of Medical Laboratory Science, College of Health Science and Technology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China[7]Institute of Artificial Intelligence Medicine, Shanghai Academy of Experimental Medicine, Shanghai, 200070, China[*1]Department of Clinical Laboratory, Xinhua Hospital, Shanghai Jiaotong University of Medicine School, Shanghai, 200092, China[*2]Department of Pathology and Laboratory Medicine, Oregon Health and Science University, Portland, OR, 97239, USA
推荐引用方式(GB/T 7714)：
Wang Weiwei,Li Haibo,Guo Yukun,et al.Immunological dynamic characteristics in acute myeloid leukemia predict the long-term outcomes and graft-versus host-disease occurrences post-transplantation[J].CLINICAL AND EXPERIMENTAL IMMUNOLOGY.2024,215(2):148-159.doi:10.1093/cei/uxad123.
APA：
Wang, Weiwei,Li, Haibo,Guo, Yukun,Zhang, Lihua,Jiang, Wenli...&Shen, Lisong.(2024).Immunological dynamic characteristics in acute myeloid leukemia predict the long-term outcomes and graft-versus host-disease occurrences post-transplantation.CLINICAL AND EXPERIMENTAL IMMUNOLOGY,215,(2)
MLA：
Wang, Weiwei,et al."Immunological dynamic characteristics in acute myeloid leukemia predict the long-term outcomes and graft-versus host-disease occurrences post-transplantation".CLINICAL AND EXPERIMENTAL IMMUNOLOGY 215..2(2024):148-159
