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Exploring the Mechanisms of Sanguinarine in the Treatment of Osteoporosis by Integrating Network Pharmacology Analysis and Deep Learning Technology

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机构: [1]Department of Orthopedics, The Sixth People's Hospital of Zhuji, Zhuji, Zhejiang, China. [2]Department of Orthopedics, Pan'an Hospital of Traditional Chinese Medicine, Jinhua, Zhejiang, China. [3]The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China. [4]Department of Orthopedics, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, China. [5]The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
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关键词: Osteoporosis network pharmacology analysis sanguinarine deep learning technology protein-protein interaction network molecular docking

摘要:
Sanguinarine (SAN) has been reported to have antioxidant, antiinflammatory, and antimicrobial activities with potential for the treatment of osteoporosis (OP).This work purposed to unravel the molecular mechanisms of SAN in the treatment of OP.OP-related genes and SAN-related targets were predicted from public databases. Differential expression analysis and VennDiagram were adopted to detect SAN-related targets against OP. Protein-protein interaction (PPI) network was served for core target identification. Molecular docking and DeepPurpose algorithm were further adopted to investigate the binding ability between core targets and SAN. Gene pathway scoring of these targets was calculated utilizing gene set variation analysis (GSVA). Finally, we explored the effect of SAN on the expressions of core targets in preosteoblastic MC3T3-E1 cells.A total of 21 candidate targets of SAN against OP were acquired. Furthermore, six core targets were identified, among which CASP3, CTNNB1, and ERBB2 were remarkably differentially expressed in OP and healthy individuals. The binding energies of SAN with CASP3, CTNNB1, and ERBB2 were -6, -6.731, and -7.162 kcal/mol, respectively. Moreover, the GSVA scores of the Wnt/calcium signaling pathway were significantly lower in OP cases than in healthy individuals. In addition, the expression of CASP3 was positively associated with Wnt/calcium signaling pathway. CASP3 and ERBB2 were significantly lower expressed in SAN group than in DMSO group, whereas the expression of CTNNB1 was in contrast.CASP3, CTNNB1, and ERBB2 emerge as potential targets of SAN in OP prevention and treatment.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.

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出版当年[2023]版:
大类 | 4 区 医学
小类 | 4 区 药物化学 4 区 计算机:跨学科应用
最新[2025]版:
大类 | 4 区 医学
小类 | 4 区 药物化学 4 区 计算机:跨学科应用
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第一作者机构: [1]Department of Orthopedics, The Sixth People's Hospital of Zhuji, Zhuji, Zhejiang, China.
通讯作者:
通讯机构: [3]The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China. [4]Department of Orthopedics, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, China. [5]The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. [*1]Department of Orthopedics, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, China [*2]The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China [*3]The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
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