Objectives: Glomerulonephritis is a serious kidney disease that can induce end-stage renal failure. The aberrant proliferation of mesangial cells is a cause of glomerulonephritis. Traditional Chinese medicines, such as Astragalus and Salvia miltrorrhiza, play important roles in the treatment of kidney-related diseases. However, the effects of a combination of Astragalus and S. miltrorrhiza-containing traditional Chinese medicines (Polygala fallax Hemsl and compound Sanqi granules) on glomerulonephritis are unclear. Methods: HRM cells (human mesangial cells) were stimulated with lipopolysaccharide to simulate glomerulonephritis. Separately, compound Sanqi granules and P. fallax Hemsl were administered to nude mice in various combinations. Serum was collected from the treated mice and added to HRM cells; the proliferation and apoptosis characteristics of the cells were assessed. Results: The proliferation of HRM cells was inhibited after exposure to serum from treated mice. Exposure to serum from treated mice moderately induced apoptosis of HRM cells and lowered the expression levels of TNF-alpha, IL-1 beta, and IL-6. Conclusions: Combination treatment with compound Sanqi granules and P. fallax Hemsl exerts a therapeutic effect on glomerulonephritis by inhibiting the proliferation of mesangial cells, while inducing apoptosis in those cells.