Chinese Herbal Formula, Huayu Tongbi Fang, Attenuates Inflammatory Proliferation of Rat Synoviocytes Induced by IL-1β by Regulating Proliferation and Differentiation of T Lymphocytes
机构:[1]Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China [2]Department of Rheumatology, (e Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510000, China 大德路总院风湿科风湿免疫研究团队广东省中医院[3]Department of Rheumatology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
The inflammatory proliferation of fibroblast-like synoviocytes (FLSs) and functional imbalances in T lymphocytes play critical roles in the pathogenesis of rheumatoid arthritis (RA). The clinical efficacy of Huayu Tongbi Fang (HYTB, a traditional herbal formula) in RA treatment has been validated. In this study, we aimed to explore the regulatory mechanisms of HYTB on the proliferation and differentiation of T lymphocytes, and the inhibitory effect of HYTB on inflammatory proliferation of FLSs. The RCS-364 (Rat FLSs) cells were cocultured with rat splenic lymphocytes that were induced by interleukin-1 beta in Transwell chambers. After freeze-dried HYTB powder treatment, the percentage of T-cell subset and apoptosis rates of FLSs were measured using flow cytometry. Furthermore, protein expression of key molecules of NF-kappa B and JAK/STAT signaling pathways was quantified using Western blot. The granulocyte-macrophage colony-stimulating factor (GM-CSF) was measured using enzyme-linked immunosorbent assay. The results showed that HYTB could inhibit the inflammatory proliferation of FLSs through inducing cell apoptosis. Additionally, HYTB treatment could intervene in the proliferation and differentiation of T lymphocytes and regulate protein expression of key molecules in NF-kappa B and JAK/STAT cell signaling pathways. Moreover, it could inhibit FLS activation by suppressing GM-CSF production by T cells and FLSs. Therefore, the HYTB formula should be used as a traditional medicine against RA in modern complementary and alternative therapies.
基金:
State Administration of Traditional Chinese Medicine; Special Project of the National Chinese Medicine Clinical Research Base [JDZX2015191]; Beijing University of Chinese Medicine [2019-JYB-XS-177]
第一作者机构:[1]Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
共同第一作者:
通讯作者:
通讯机构:[1]Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China [3]Department of Rheumatology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
推荐引用方式(GB/T 7714):
Chen Hening,Wang Changzhi,Li Jinyu,et al.Chinese Herbal Formula, Huayu Tongbi Fang, Attenuates Inflammatory Proliferation of Rat Synoviocytes Induced by IL-1β by Regulating Proliferation and Differentiation of T Lymphocytes[J].EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE.2020,2020:doi:10.1155/2020/1706837.
APA:
Chen, Hening,Wang, Changzhi,Li, Jinyu,Huandike, Meiyier,Liu, Juan...&Chai, Lenin.(2020).Chinese Herbal Formula, Huayu Tongbi Fang, Attenuates Inflammatory Proliferation of Rat Synoviocytes Induced by IL-1β by Regulating Proliferation and Differentiation of T Lymphocytes.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2020,
MLA:
Chen, Hening,et al."Chinese Herbal Formula, Huayu Tongbi Fang, Attenuates Inflammatory Proliferation of Rat Synoviocytes Induced by IL-1β by Regulating Proliferation and Differentiation of T Lymphocytes".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2020.(2020)