机构：[1]Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou,[2]Department of Anesthesiology, Nanfang Hospital, Southern Medical University, Guangzhou,[3]The Qingyuan traditional Chinese medical hospital of Guangdong Provence, China[4]Department of Clinical Laboratory, Guangzhou Twelfth People’s Hospital, Guangzhou, China[5]Department of Vascular Surgery, Nanfang Hospital, Southern Medical University, Guangzhou,[6]Department of Cardiovascular Sciences, University of Leicester, Leicester, UK[7]Shantou University Medical College, Shantou, China[8]Laboratory Medicine Center, Guangzhou Women and Children's Medical Center, Guangzhou 510000,
Long noncoding (lnc)RNAs have been implicated in the development and progression of atherosclerosis. However, the expression and mechanism of action of lncRNAs in atherosclerosis are still unclear. We implemented microarray analysis in human advanced atherosclerotic plaques and normal arterial intimae to detect the lncRNA and mRNA expression profile. Gene Ontology functional enrichment and pathway analyses were applied to explore the potential functions and pathways involved in the pathogenesis of atherosclerosis. A total of 236 lncRNAs and 488 mRNAs were selected for further Ingenuity Pathway Analysis. Moreover, quantitative RT-PCR tests of most selected lncRNAs and mRNAs with high fold changes were consistent with the microarray data. We also performed ELISA to investigate the corresponding proteins levels of selected genes and showed that serum levels of SPP1, CD36, ATP6V0D2, CHI3L1. MYH11, and BDNF were differentially expressed in patients with coronary heart disease compared with healthy subjects. These proteins correlated with some biochemical parameters used in the diagnosis of cardiovascular diseases. Furthermore, receiver operating characteristic analysis showed a favorable diagnostic performance. The microarray profiling analysis and validation of differentially-expressed lncRNAs and mRNAs in atherosclerosis not only provide new insights into the pathogenesis of this disease but may also reveal new biomarkers for its diagnosis and treatment.
基金：
National Natural Science Foundation of China [81871701, 81572051, 81772244]; Natural Science Fund of GuangdongNational Natural Science Foundation of Guangdong Province [2017A030313535, 2017A030313532, 2018A030313533]; Science and Technology Program of Guangzhou [201607010267, 201604020015, 201707010034, 201704020213, 201707010156]; Foundation of Qingyuan Science and Technology Bureau Project [2018A009]; Guangdong Province Medical Science and Technology Research Fund [A2015009]
第一作者机构：[1]Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou,
共同第一作者：
通讯作者：
通讯机构：[1]Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou,[8]Laboratory Medicine Center, Guangzhou Women and Children's Medical Center, Guangzhou 510000,[*1]Laboratory Medicine Center, Nanfang Hospital,Southern Medical University, Guangzhou, Guangdong 510515, China.
推荐引用方式(GB/T 7714)：
Bai Huan-Lan,Lu Zhi-Feng,Zhao Jing-Jing,et al.Microarray profiling analysis and validation of novel long noncoding RNAs and mRNAs as potential biomarkers and their functions in atherosclerosis[J].PHYSIOLOGICAL GENOMICS.2019,51(12):644-656.doi:10.1152/physiolgenomics.00077.2019.
APA：
Bai, Huan-Lan,Lu, Zhi-Feng,Zhao, Jing-Jing,Ma, Xin,Li, Xue-Heng...&Hu, Yan-Wei.(2019).Microarray profiling analysis and validation of novel long noncoding RNAs and mRNAs as potential biomarkers and their functions in atherosclerosis.PHYSIOLOGICAL GENOMICS,51,(12)
MLA：
Bai, Huan-Lan,et al."Microarray profiling analysis and validation of novel long noncoding RNAs and mRNAs as potential biomarkers and their functions in atherosclerosis".PHYSIOLOGICAL GENOMICS 51..12(2019):644-656
