机构：[1]Department of Plastic Surgery, People’s Hospital of Zhongshan City, Zhongshan, Guangdong, China,[2]Department of Plastic Surgery, General Hospital of Southern Theater Command, PLA, Guangzhou, Guangdong, China,[3]The Key Laboratory of Trauma Treatment and Tissue Repair of Tropical Area, PLA, Guangzhou, Guangdong, China,[4]Graduate School, Shantou University Medical College, Shantou, Guangdong, China, and[5]Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, Guangdong, China广东省中医院
In recent years, autologous platelet-rich plasma (PRP) derivatives have been used widely in the regeneration and repair of tissue, but a standard definition and preparation method for PRP are lacking. We developed a standardized method using platelet indices as quality-control indicators for PRP preparation. Twenty-one elderly patients (9 males, 12 females) with complex wounds were treated with standardized platelet-rich plasma (S-PRP). The platelet count in PRP after the second centrifugation was 1,069-1,436x10(9)/L. We adjusted the platelet concentration in PRP after a second centrifugation to 1,000x10(9)/L according to a formula using platelet-poor plasma (PPP). The standardized preparation method that we developed gave S-PRP with a relatively uniform platelet concentration. The wounds of 21 patients showed accelerated healing after S-PRP treatment, and there were no obvious side effects during treatment. These data suggest that our preparation method of S-PRP, using platelet indices as quality-control indicators with platelet count of 1,000x10(9)/L could be used for the treatment of complex wounds in the elderly. The preparation method of S-PRP proposed in the present study may be a simple and effective method of PRP quality control.
基金：
the
National Natural Science Foundation of China (81,671,924,
81,272,105), National Key Research and Development Plan
of China (2017YFC1103301), National Basic Science and
Development Program (973 Program) (2012CB518105),
Health and Medical Treatment Collaborative Innovation
Major Special Projects of Guangzhou (201508020253), Science
and Technology Planning Project of Guangdong Province
of China (2015B020233012), and the Science and
Technology Key Project of Guangdong Province
(2014B020212010).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区医学
小类|2 区皮肤病学3 区医学：研究与实验3 区外科4 区细胞生物学
最新[2025]版：
大类|3 区医学
小类|3 区细胞生物学3 区皮肤病学3 区医学：研究与实验3 区外科
JCR分区：
出版当年[2017]版：
Q1SURGERYQ1DERMATOLOGYQ2MEDICINE, RESEARCH & EXPERIMENTALQ3CELL BIOLOGY
最新[2023]版：
Q1DERMATOLOGYQ1SURGERYQ2CELL BIOLOGYQ2MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Department of Plastic Surgery, People’s Hospital of Zhongshan City, Zhongshan, Guangdong, China,[2]Department of Plastic Surgery, General Hospital of Southern Theater Command, PLA, Guangzhou, Guangdong, China,[3]The Key Laboratory of Trauma Treatment and Tissue Repair of Tropical Area, PLA, Guangzhou, Guangdong, China,
共同第一作者：
通讯作者：
通讯机构：[2]Department of Plastic Surgery, General Hospital of Southern Theater Command, PLA, Guangzhou, Guangdong, China,[3]The Key Laboratory of Trauma Treatment and Tissue Repair of Tropical Area, PLA, Guangzhou, Guangdong, China,[*1]General Hospital of Southern Theater Command, People’s Liberation Army, 111 Guangzhou Liu Hua Road, Guangzhou, Guangdong 510010, China
推荐引用方式(GB/T 7714)：
Tian Ju,Cheng Liu-Hang-Hang,Cui Xiao,et al.Application of standardized platelet-rich plasma in elderly patients with complex wounds[J].WOUND REPAIR AND REGENERATION.2019,27(3):268-276.doi:10.1111/wrr.12702.
APA：
Tian, Ju,Cheng, Liu-Hang-Hang,Cui, Xiao,Lei, Xiao-Xuan,Tang, Jian-Bing&Cheng, Biao.(2019).Application of standardized platelet-rich plasma in elderly patients with complex wounds.WOUND REPAIR AND REGENERATION,27,(3)
MLA：
Tian, Ju,et al."Application of standardized platelet-rich plasma in elderly patients with complex wounds".WOUND REPAIR AND REGENERATION 27..3(2019):268-276
