机构：[1]Reproductive medical center, Guangdong Women and Children Hospital, Guangzhou Medical University, 511400,Guangzhou, China[2]Clinical laboratory, Traditional Chinese Medicine Hospital of Guangdong province, 510120,Guangzhou, China广东省中医院[3]Department of Pathogenic Biology and Immunology, Sino-French Hoffmann Institute,Guangzhou Medical University, 511436, Guangzhou, China[4]The Sixth Affiliated Hospital of Sun Yat-Sen University,510655, Guangzhou, China中山大学附属第六医院[5]State Key Laboratory of Respiratory Disease, Guangzhou Institute of RespiratoryHealth, The First Affiliated Hospital of Guangzhou Medical University, 510120, Guangzhou, China
Immune responses play an important role in the pathogenesis of polycystic ovary syndrome (PCOS). However, the characteristics of T lymphocyte subsets in PCOS remain insufficiently understood. In this study, lymphocytes of follicular fluid (FF) were obtained from oocyte retrieval before in-vitro fertilization (IVF) in infertile women with or without PCOS. The levels of cluster of differentiation 25 (CD25), CD69, programmed death 1 (PD-1), interferon-gamma (IFN-gamma), interleukin 17A (IL-17A) and IL-10 in T lymphocytes were determined by flow cytometry. Our results showed that the percentage of FF CD8(+) T cells was significantly decreased in infertile patients with PCOS (P < 0.05). Furthermore, the levels of CD69 and IFN-gamma were significantly decreased and the level of PD-1 was increased in both CD4(+) and CD8(+) T cells from infertile patients with PCOS (P < 0.05). Moreover, the expression of PD-1 on CD4(+) or CD8(+) T cells was positively correlated with the estradiol (E2) levels in the serum and reversely correlated with the expression of IFN-gamma in CD4(+) or CD8(+) T cells in infertile patients with PCOS. These results suggested that T cell dysfunction may be involved in the pathogenesis of PCOS.
基金：
Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2016A030310282, 2018A0303130317]; Natural Science Foundation of ChinaNational Natural Science Foundation of China [31800739, 81771696]; Guangdong provincial education department [2016KZDXM033, 2017KTSCX157]; Science and Technology Planning Project of Guangdong Province [2016A020215164]
第一作者机构：[1]Reproductive medical center, Guangdong Women and Children Hospital, Guangzhou Medical University, 511400,Guangzhou, China
共同第一作者：
通讯作者：
通讯机构：[1]Reproductive medical center, Guangdong Women and Children Hospital, Guangzhou Medical University, 511400,Guangzhou, China[3]Department of Pathogenic Biology and Immunology, Sino-French Hoffmann Institute,Guangzhou Medical University, 511436, Guangzhou, China[5]State Key Laboratory of Respiratory Disease, Guangzhou Institute of RespiratoryHealth, The First Affiliated Hospital of Guangzhou Medical University, 510120, Guangzhou, China
推荐引用方式(GB/T 7714)：
Zitao Li,Anping Peng,Yuanfa Feng,et al.Detection of T lymphocyte subsets and related functional molecules in follicular fluid of patients with polycystic ovary syndrome[J].SCIENTIFIC REPORTS.2019,9:-.doi:10.1038/s41598-019-42631-x.
APA：
Zitao Li,Anping Peng,Yuanfa Feng,Xiaona Zhang,Fenghua Liu...&Quan Yang.(2019).Detection of T lymphocyte subsets and related functional molecules in follicular fluid of patients with polycystic ovary syndrome.SCIENTIFIC REPORTS,9,
MLA：
Zitao Li,et al."Detection of T lymphocyte subsets and related functional molecules in follicular fluid of patients with polycystic ovary syndrome".SCIENTIFIC REPORTS 9.(2019):-
