机构：[1]Department of Neurology, GuangDong Province Hospital Of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China广东省中医院[2]Department of Neurology, Liuzhou Traditional ChineseMedical Hospital, the Third Affiliated Hospital of Guangxi University of Chinese Medicine, Liuzhou , China[3]Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China
The aberrant expression of lncRNAs has been inferred to be closely related with the progression of neural ischemia/reperfusion (I/R) injury. RMRP is an lncRNA associated with I/R injury. In order to determine the role of RMRP in I/R injury, the effects of RMRP knockdown on oxygen-glucose deprivation/re-oxygenation (OGD/R)-induced injury in SH-SY5Y cells were evaluated. The effect of OGD/R administration on the expression of RMRP and apoptosis in SH-SY5Y cells, and the effect of RMRP suppression by siRNA on the impairments of cells proliferation and mobility potential due to OGD/R administration were assessed in the current study. At the molecular level, the current study detected the expressions of indicators involved in autophagy and PI3K/Akt/mTOR-mediated apoptosis pathways. The OGD/R administration induced the expression of RMRP and apoptosis in SH-SY5Y cells. After RMRP knockdown, the proliferation potential of SH-SY5Y cells was restored, and apoptosis and cell cycle arrest were inhibited. Moreover, RMRP inhibition also increased the invasion and migration of SH-SY5Y cells which were treated with OGD/R. The effects of RMRP suppression on the phenotypes of SH-SY5Y were associated with the inhibition of LC3II, p-PI3K, p-Akt, and p-mTOR as well as the induction of P62 and Bcl-2. Inhibition of RMRP contributed to the improvement of OGD/R-induced neuronal injury, which might be mediated through the inhibition of autophagy and apoptosis pathways.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81760902, 81673950]; Science and Technology Innovation Ability and the Condition of Construction Project of Liuzhou [2016G020213]; Guangdong Provincial Science and Technology Plan Projects [2016A020215101, 2017A020215182]; 2016 Specific Research Fund for TCM Science and Technology of Guangdong Provincial Hospital of Chinese Medicine [YN2016ZD04]
第一作者机构：[1]Department of Neurology, GuangDong Province Hospital Of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China[2]Department of Neurology, Liuzhou Traditional ChineseMedical Hospital, the Third Affiliated Hospital of Guangxi University of Chinese Medicine, Liuzhou , China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurology, GuangDong Province Hospital Of Traditional Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China[3]Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China[*1]Guangdong Province Hospital Of Traditional Chinese Medicine, 111# DA DE Road, Guangzhou 510120, Guangdong Province, China[*2]Department of Neurology, Nanfang Hospital, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, Guangdong 510515, China
推荐引用方式(GB/T 7714)：
Zhou Zheyi,Xu Hong,Liu Baozhu,et al.Suppression of lncRNA RMRP ameliorates oxygen-glucose deprivation/re-oxygenation-induced neural cells injury by inhibiting autophagy and PI3K/Akt/mTOR-mediated apoptosis[J].BIOSCIENCE REPORTS.2019,39:doi:10.1042/BSR20181367.
APA：
Zhou, Zheyi,Xu, Hong,Liu, Baozhu,Dun, Linglu,Lu, Changjun...&Wang, Honghao.(2019).Suppression of lncRNA RMRP ameliorates oxygen-glucose deprivation/re-oxygenation-induced neural cells injury by inhibiting autophagy and PI3K/Akt/mTOR-mediated apoptosis.BIOSCIENCE REPORTS,39,
MLA：
Zhou, Zheyi,et al."Suppression of lncRNA RMRP ameliorates oxygen-glucose deprivation/re-oxygenation-induced neural cells injury by inhibiting autophagy and PI3K/Akt/mTOR-mediated apoptosis".BIOSCIENCE REPORTS 39.(2019)
