机构：[1]Department of Traditional Chinese Medicine, Shenzhen People’s Hospital (Second Clinical Medical College of Jinan University, First Affiliated Hospital of Southern University of Science and Technology), NO. 1017 Dongmen North Road, Luohu District, Shenzhen, Guangdong Province, China, 518020深圳市康宁医院深圳市人民医院深圳医学信息中心中国医科大学附属盛京医院[2]School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, NO. 280 Daxuecheng Outer Ring East Road, Guangzhou, Guangdong Province, China, 510006[3]Microbiology Laboratory, Shenzhen Institute for Drug Control, No. 28 Gaoxin Middle Second Road, Nanshan District, Shenzhen City, Guangdong Province, China, 518057
This study was aimed to investigate the anti-tumor activity of rubimaillin in vitro, and the mechanism involved. The inhibitory effect of rubimaillin on cell proliferation was determined with MTT assay. Apoptosis was assayed using AV/PI double staining, while the mitochondrial membrane potential of SKOV-3 cells was determined with Rhodamine 123 (Rh123) staining. Western blot assay was used to determine the effect of rubimaillin on the expressions of Bcl-2, Bax, PARP, cleaved PARP, caspase3, cleaved caspase3, and other apoptosis-related proteins in SKOV-3 cells. Rubimaillin inhibited the growth of SKOV-3 cells in a concentration-dependent manner and induced apoptosis of tumor cells through the mitochondrial apoptosis pathway. These results indicate that rubimaillin is a potential anti-ovarian cancer drug.
基金：
Second batch of Smriti Project of Famous Traditional Chinese Medicine Doctor in Guangdong Province in 2015, (Guangdong Province traditional Chinese medicine [2015]) [93]; Funds of Shenzhen Special Specialty Project of Traditional Chinese Medicine in 2018. (Shenzhen Municipal Health Commissiom traditional Chinese medicine [2018]) [51]
第一作者机构：[1]Department of Traditional Chinese Medicine, Shenzhen People’s Hospital (Second Clinical Medical College of Jinan University, First Affiliated Hospital of Southern University of Science and Technology), NO. 1017 Dongmen North Road, Luohu District, Shenzhen, Guangdong Province, China, 518020
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Siwei,Wu Zhen,Zhou Zhi,et al.Rubimaillin decreases the viability of human ovarian cancer cells via mitochondria-dependent apoptosis[J].CELLULAR AND MOLECULAR BIOLOGY.2019,65(7):72-76.doi:10.14715/cmb/2019.65.7.13.
APA：
Zhang Siwei,Wu Zhen,Zhou Zhi,Hu Xuguang&Li Yousheng.(2019).Rubimaillin decreases the viability of human ovarian cancer cells via mitochondria-dependent apoptosis.CELLULAR AND MOLECULAR BIOLOGY,65,(7)
MLA：
Zhang Siwei,et al."Rubimaillin decreases the viability of human ovarian cancer cells via mitochondria-dependent apoptosis".CELLULAR AND MOLECULAR BIOLOGY 65..7(2019):72-76
