机构：[1]The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong, China广东省中医院[2]Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, Guangdong, China广东省中医院[3]Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, Guangdong, China广东省中医院[4]Dermatology Department, Guangdong Hospital of Traditional Chinese Medicine, Guangzhou 510120, Guangdong, China大德路总院皮肤科大德路总院皮肤科广东省中医院[5]Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangzhou 510120, Guangdong, China
Background: Psoriasis is an inflammatory skin disease that affects an estimated 3% of the world's population. PSORI-CM02 is an empirically developed Chinese medicine formula optimised from Yin Xie Ling, summarised by national medical master Guo-Wei Xuan, that has been used for decades to treat psoriasis in the Guangdong Provincial Hospital of Chinese Medicine. However, its anti-psoriatic mechanisms are still poorly understood. In this study, we explored the effects of PSORI-CM02 on autophagy and the underlying mechanisms in TNF-alpha-stimulated HaCaT cells and in a mouse model of imiquimod-induced psoriasis. Methods: Cell viability was assessed by MTT assay. Apoptosis was detected by annexin V-FITC/PI double-staining and caspase-3 assays. Autophagy was detected by electron microscopy, RT-PCR and western blotting. The PI3K/Akt/mTOR pathway was analysed by western blotting and immunochemical analysis. Results: PSORI-CM02 induced autophagy and thus inhibited the proliferation of HaCaT cells via suppression of the PI3K/Akt/mTOR pathway. In mice with IMQ-induced psoriasis, PSORI-CM02 relieved psoriasis symptoms, induced autophagy and inhibited the phosphorylation of the PI3K/AKT/mTOR pathway in the skin. Conclusion: These results suggest that PSORI-CM02 treats psoriasis by inducing autophagy via inhibition of the PI3K/Akt/mTOR pathway.
基金：
National Natural
Science Foundation of China (81803804); the Guangdong Province
Natural Science Fund (S2013030011515, 2017A030310124); the
Guangdong Province Science and Technology Planning Project
(2017A050506041, 2017B030314166); the Guangdong Provincial
Department of Education Project (2018KQNCX046); the Guangzhou
Science and Technology Project (201807010051) and the Guangdong
Provincial Hospital of Chinese Medicine Special Fund (YN2016ZD01,
YN2018HK01, YN2018ZD08, YN2018RBA02, YN0216XP02).
基金编号：81803804
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区医学
小类|1 区全科医学与补充医学2 区药物化学2 区药学2 区植物科学
最新[2025]版：
大类|1 区医学
小类|1 区药物化学1 区全科医学与补充医学1 区药学1 区植物科学
JCR分区：
出版当年[2017]版：
Q1PLANT SCIENCESQ1PHARMACOLOGY & PHARMACYQ1CHEMISTRY, MEDICINALQ1INTEGRATIVE & COMPLEMENTARY MEDICINE
第一作者机构：[1]The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong, China[2]Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, Guangdong, China[3]Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, Guangdong, China[4]Dermatology Department, Guangdong Hospital of Traditional Chinese Medicine, Guangzhou 510120, Guangdong, China
通讯作者：
通讯机构：[1]The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou 510120, Guangdong, China[2]Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, Guangdong, China[3]Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510120, Guangdong, China[4]Dermatology Department, Guangdong Hospital of Traditional Chinese Medicine, Guangzhou 510120, Guangdong, China[5]Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangzhou 510120, Guangdong, China
推荐引用方式(GB/T 7714)：
Lu Yue,Wang Ailin,Zhang Jinwei,et al.PSORI-CM02 ameliorates psoriasis in vivo and in vitro by inducing autophagy via inhibition of the PI3K/Akt/mTOR pathway[J].PHYTOMEDICINE.2019,64:doi:10.1016/j.phymed.2019.153054.
APA：
Lu Yue,Wang Ailin,Zhang Jinwei,Li Leng,Wei Jianan...&Lu Chuanjian.(2019).PSORI-CM02 ameliorates psoriasis in vivo and in vitro by inducing autophagy via inhibition of the PI3K/Akt/mTOR pathway.PHYTOMEDICINE,64,
MLA：
Lu Yue,et al."PSORI-CM02 ameliorates psoriasis in vivo and in vitro by inducing autophagy via inhibition of the PI3K/Akt/mTOR pathway".PHYTOMEDICINE 64.(2019)
医学