机构：[1]Department of Central Laboratory, Shenzhen Hospital, Beijing University of Chinese Medicine, Shenzhen University School of Medicine, Shenzhen 518100, Guangdong, China北京大学深圳医院深圳市康宁医院深圳医学信息中心[2]Institute of Translational Medicine, Shenzhen Second People’s Hospital, First A.liated Hospital of Shenzhen University, Shenzhen 518035, China[3]Zhongshan School of Medicine, Shenzhen Second People’s Hospital, Guangzhou 510080, China深圳医学信息中心中国医学科学院阜外医院深圳医院
Caterpillar fungus is a well-known fungal Chinese medicine. To reveal molecular changes during early and late stages of adenosine biosynthesis, transcriptome analysis was performed with the anamorph strain of caterpillar fungus. A total of 2,764 differentially expressed genes (DEGs) were identified (p <= 0.05, |log(2) Ratio| >= 1), of which 1,737 were up-regulated and 1,027 were down-regulated. Gene expression profiling on 4-10 d revealed a distinct shift in expression of the purine metabolism pathway. Differential expression of 17 selected DEGs which involved in purine metabolism (map00230) were validated by qPCR, and the expression trends were consistent with the RNA-Seq results. Subsequently, the predicted adenosine biosynthesis pathway combined with qPCR and gene expression data of RNA-Seq indicated that the increased adenosine accumulation is a result of down-regulation of ndk, ADK, and APRT genes combined with up-regulation of AK gene. This study will be valuable for understanding the molecular mechanisms of the adenosine biosynthesis in caterpillar fungus.
基金：
is study was supported by National Natural Science
Foundation of China (81803652), Natural Science Foundation
of Guangdong Province (2018A0303100007), Foundation of
Health and Family Planning Commission (SZBC2018016),
China Postdoctoral Science Foundation (2018M633261).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区生物
小类|3 区生物工程与应用微生物4 区医学：研究与实验
最新[2025]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区医学：研究与实验
JCR分区：
出版当年[2017]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Department of Central Laboratory, Shenzhen Hospital, Beijing University of Chinese Medicine, Shenzhen University School of Medicine, Shenzhen 518100, Guangdong, China[2]Institute of Translational Medicine, Shenzhen Second People’s Hospital, First A.liated Hospital of Shenzhen University, Shenzhen 518035, China[3]Zhongshan School of Medicine, Shenzhen Second People’s Hospital, Guangzhou 510080, China
通讯作者：
通讯机构：[1]Department of Central Laboratory, Shenzhen Hospital, Beijing University of Chinese Medicine, Shenzhen University School of Medicine, Shenzhen 518100, Guangdong, China[2]Institute of Translational Medicine, Shenzhen Second People’s Hospital, First A.liated Hospital of Shenzhen University, Shenzhen 518035, China[3]Zhongshan School of Medicine, Shenzhen Second People’s Hospital, Guangzhou 510080, China
推荐引用方式(GB/T 7714)：
Shan Lin,Zhicheng Zou,Cuibing Zhou,et al.Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus[J].BIOMED RESEARCH INTERNATIONAL.2019,2019:doi:10.1155/2019/1864168.
APA：
Shan Lin,Zhicheng Zou,Cuibing Zhou,Hancheng Zhang&Zhiming Cai.(2019).Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus.BIOMED RESEARCH INTERNATIONAL,2019,
MLA：
Shan Lin,et al."Transcriptome Analysis Reveals the Molecular Mechanisms Underlying Adenosine Biosynthesis in Anamorph Strain of Caterpillar Fungus".BIOMED RESEARCH INTERNATIONAL 2019.(2019)
