机构：[1]Department of Anesthesiology, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510010, Guangdong, China[2]Department of Anesthesiology, Second Affiliated Hospital, Guangzhou University of TCM, Guangzhou 510120, Guangdong, China
We constructed a water-soluble lipopolymer (WSLP) as a nonviral gene carrier to deliver siRNA targeting NR2B. The cytotoxicity and serum stability of WSLP loaded with siRNA were evaluated, and the knockdown efficiency of WSLP/NR2B-siRNA in PC12 cells was examined. The results showed that WSLP could protect the loading siRNAs from enzymatic degradation in serum and exhibit low cytotoxicity to cells. After transfection, WSLP/NR2B-siRNA complexes reduced the NR2B transcriptional level by 50% and protein level by 55% compared to control siRNA. Moreover, 3 days after intrathecal injection of WSLP/NR2B-siRNA complexes into rats, the NR2B protein expression decreased significantly to 58%, compared to control treatment (p < 0.01). Injection of WSLP with scrambled siRNA or of polyethylenimine (PEI) with NR2B-siRNA did not show this inhibitory effect. Additionally, injection of WSLP/NR2B-siRNA complexes significantly relieved inflammatory pain in rats at 3, 4, and 5 days with reduced MWT and decreased TWL scores, while injection of WSLP with scrambled siRNA or of PEI with NR2B-siRNA did not. These results demonstrated that WSLP can efficiently deliver siRNA targeting NR2B to PC12 cells and relieve pain in rats with chronic inflammatory pain.
基金：
National Natural
Science Foundation of China (nos. 81100817 and 81371233)
and Science and Technology Project of Guangdong Province
(no. 2014A020212554).
第一作者机构：[1]Department of Anesthesiology, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510010, Guangdong, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Peng Jie,Ma Jiahui,Yang Xue,et al.Water-Soluble Polymer Assists N-Methyl-D-Aspartic Acid Receptor 2B siRNA Delivery to Relieve Chronic Inflammatory Pain In Vitro and In Vivo[J].PAIN RESEARCH & MANAGEMENT.2018,2018:doi:10.1155/2018/7436060.
APA：
Peng, Jie,Ma, Jiahui,Yang, Xue,He, Huan,Wu, Haopeng...&Lu, Jianhua.(2018).Water-Soluble Polymer Assists N-Methyl-D-Aspartic Acid Receptor 2B siRNA Delivery to Relieve Chronic Inflammatory Pain In Vitro and In Vivo.PAIN RESEARCH & MANAGEMENT,2018,
MLA：
Peng, Jie,et al."Water-Soluble Polymer Assists N-Methyl-D-Aspartic Acid Receptor 2B siRNA Delivery to Relieve Chronic Inflammatory Pain In Vitro and In Vivo".PAIN RESEARCH & MANAGEMENT 2018.(2018)
