机构：[1]Department of Traditional Chinese Medicine, Beijing Shijitan Hospital, Capital Medical University, 100038 Beijing, China[2]Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, 100094 Beijing, China[3]Materia Medica of China Academy of Chinese Medical Sciences, 100700 Beijing, China[4]Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, 510120 Guangzhou, China广东省中医院深圳市中医院深圳医学信息中心
Background and Objective: Stellera chamaejasme L., was found to have anti-tumor effects. This study aimed to investigate the anti-proliferative effects of the ethanol extract of S. chamaejasme L. (EES), on human Hepatocellular Carcinoma (HC) cell line and explore the mechanism of EES inhibit HC cell proliferation. Methodology: In this study 3-(4,5)-dimethylthiazol (-z-y1)-3,5-di-phenyltetrazoliumbromide(MTT)assaywas used to assess the viability of tumor cells submitted to EES. Application of luciferase report gene and Western Blotting (WB) were employed to investigate transforming growth factor beta(TGF-beta), Smad4 and p-Akt expression. Results: The EES significantly decreased hepatoma cell (HepG2, BEL-7402, SMMC-7721) proliferation in a dose-dependent manner with an IC50 37.75, 28.60 and 29.22 mu g mL(-1) in vitro (p<0.05). The EES could strongly inhibit TGF-beta and down-regulated its downstream factors, such as T beta R-1, Smad4, p-Akt in protein level. Conclusion: The EES decreased hepatoma cell proliferation via down-regulation Smad4-mediated TGF-beta signaling pathway.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81001564]; Beijing Nova ProgramBeijing Municipal Science & Technology Commission [Z141107001814061]; Beijing Municipality Health Technology High level Talent [2014-3-046]; Research Project of Beijing Integrative Medicine Institute of Cancer; Construction Project of Beijing Integrative Medicine Treatment Center of Cancer
第一作者机构：[1]Department of Traditional Chinese Medicine, Beijing Shijitan Hospital, Capital Medical University, 100038 Beijing, China
通讯作者：
通讯机构：[1]Department of Traditional Chinese Medicine, Beijing Shijitan Hospital, Capital Medical University, 100038 Beijing, China[4]Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, 510120 Guangzhou, China[*1]Department of Traditional Chinese Medicine, Beijing Shijitan Hospital, Capital Medical University, 100038 Beijing, China[*2]Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, 510120 Guangzhou, China
推荐引用方式(GB/T 7714)：
Pan Guofeng,Cheng Long,Feng Xingzhong,et al.Ethanol Extract of Stellera chamaejasme L Inhibits Hepatoma Cell Proliferation Through Down-regulation of Smad4-mediated TGF-β Signaling Pathway[J].INTERNATIONAL JOURNAL OF PHARMACOLOGY.2017,13(6):628-635.doi:10.3923/ijp.2017.628.635.
APA：
Pan, Guofeng,Cheng, Long,Feng, Xingzhong,Zhu, Xiaoxin&Wu, Guangping.(2017).Ethanol Extract of Stellera chamaejasme L Inhibits Hepatoma Cell Proliferation Through Down-regulation of Smad4-mediated TGF-β Signaling Pathway.INTERNATIONAL JOURNAL OF PHARMACOLOGY,13,(6)
MLA：
Pan, Guofeng,et al."Ethanol Extract of Stellera chamaejasme L Inhibits Hepatoma Cell Proliferation Through Down-regulation of Smad4-mediated TGF-β Signaling Pathway".INTERNATIONAL JOURNAL OF PHARMACOLOGY 13..6(2017):628-635
