机构：[1]College of Food and Bioengineering, South China University of Technology, Guangzhou, 510640, China.[2]College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China[3]The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510405, China深圳市中医院深圳医学信息中心[4]The second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, China.广东省中医院深圳市中医院深圳医学信息中心
Rubus chingii Hu, namely "Fu-pen-zi" in Chinese, has been used as a functional food in China for a long time. This study aims to identify its bioactive constituents with antioxidant and anti-tumor properties. R. chingii was extracted with 95% ethanol and then partitioned into four fractions: petroleum ether fraction, ethyl acetate fraction, n-butanol fraction, and water fraction. Results showed that the ethyl acetate fraction had the strongest antioxidant activity and cytotoxicity against human cancer cell lines (HepG-2, Bel-7402, A549 and MCF-7). Therefore, four compounds were isolated from the ethyl acetate fraction, and they were identified as ent-16 alpha,17-dihydroxy-kauran-19-oic acid, tormentic acid, oleanolic acid and beta-daucosterol, the first two of which were isolated and identified from R. chingii for the first time. In particular, tormentic acid exhibited excellent cytotoxicity activities against human tumor cell lines. The results obtained in this work might contribute to the understanding of biological activities of R. chingii and further investigation on its potential application is valued for food and drugs.
基金：
National Natural Foundation of ChinaNational Natural Science Foundation of China [31301453]
第一作者机构：[1]College of Food and Bioengineering, South China University of Technology, Guangzhou, 510640, China.[2]College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Tian-Tian,Liu Ya-Jun,Yang Li,et al.Extraction of antioxidant and antiproliferative ingredients from fruits of Rubus chingii Hu by active tracking guidance[J].MEDCHEMCOMM.2017,8(8):1673-1680.doi:10.1039/c7md00240h.
APA：
Zhang, Tian-Tian,Liu, Ya-Jun,Yang, Li,Jiang, Jian-Guo,Zhao, Jing-Wen&Zhu, Wei.(2017).Extraction of antioxidant and antiproliferative ingredients from fruits of Rubus chingii Hu by active tracking guidance.MEDCHEMCOMM,8,(8)
MLA：
Zhang, Tian-Tian,et al."Extraction of antioxidant and antiproliferative ingredients from fruits of Rubus chingii Hu by active tracking guidance".MEDCHEMCOMM 8..8(2017):1673-1680
