机构:[1]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China[2]Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China[3]Agilent Technologies, 5301 Stevens Creek Blvd, Santa Clara, CA 95051, USA[4]Key Laboratory of Medical Molecular Virology of Ministries of Education andHealth, Basic Medical College, Fudan University, Shanghai 200032, China[5]Lindsley F Kimball Research Institute, New York Blood Center, NY 10065, USA[6]Faculty of Information Technology, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China[7]Department of Rheumatologyand Immunology, Peking University People’s Hospital, 11 Xizhimen South Street, Beijing 100044, China[8]Division of Rheumatology, Jiujiang First People’sHospital, Taling North Road 48, Jiujiang 332000, China[9]Agilent Technologies Hong Kong Ltd.,Suite 2603, 26/F, AXA Tower, Landmark East, Kwun Tong,Hong Kong, China[10]Guangdong Provincial Hospital of Chinese Medicine, Dade Road 111, Guangzhou 510120, China大德路总院广东省中医院
N-linked glycans on immunoglobulin G (IgG) have been associated with pathogenesis of diseases and the therapeutic functions of antibody-based drugs; however, low-abundance species are difficult to detect. Here we show a glycomic approach to detect these species on human IgGs using a specialized microfluidic chip. We discover 20 sulfated and 4 acetylated N-glycans on IgGs. Using multiple reaction monitoring method, we precisely quantify these previously undetected low-abundance, trace and even ultra-trace N-glycans. From 277 patients with rheumatoid arthritis (RA) and 141 healthy individuals, we also identify N-glycan biomarkers for the classification of both rheumatoid factor (RF)-positive and negative RA patients, as well as anti-citrullinated protein antibodies (ACPA)-positive and negative RA patients. This approach may identify N-glycosylation-associated biomarkers for other autoimmune and infectious diseases and lead to the exploration of promising glycoforms for antibody therapeutics.
基金:
Macao Science and Technology Development Fund [020/2013/A1, 023/2016/AFJ]
第一作者机构:[1]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China[2]Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China
通讯作者:
通讯机构:[1]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China[2]Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China
推荐引用方式(GB/T 7714):
Wang Jing-Rong,Gao Wei-Na,Grimm Rudolf,et al.A method to identify trace sulfated IgG N-glycans as biomarkers for rheumatoid arthritis[J].NATURE COMMUNICATIONS.2017,8:doi:10.1038/s41467-017-00662-w.
APA:
Wang, Jing-Rong,Gao, Wei-Na,Grimm, Rudolf,Jiang, Shibo,Liang, Yong...&Jiang, Zhi-Hong.(2017).A method to identify trace sulfated IgG N-glycans as biomarkers for rheumatoid arthritis.NATURE COMMUNICATIONS,8,
MLA:
Wang, Jing-Rong,et al."A method to identify trace sulfated IgG N-glycans as biomarkers for rheumatoid arthritis".NATURE COMMUNICATIONS 8.(2017)