机构：[1]The Research Centre for Integrative Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.广东省中医院[2]Integrative Research Labrotary of Breast Cancer, Discipline of Integrated Chinese and Western Medicine, The second afliated hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China[3]Post-Doctoral Research Center, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China[4]School of Chinese Medicine, the University of Hong Kong, Pok Fu Lam, SAR, Hong Kong
Network pharmacology has become a powerful means of understanding the mechanisms underlying the action of Chinese herbs in cancer treatment. This study aims to validate the preventive effects and molecular mechanisms of a clinical prescription XIAOPI formula against breast cancer. In vivo breast cancer xenograft data showed that XIAOPI delayed breast cancer development and efficiently inhibited lung metastasis, accompanied by prolonged survival benefits and decreased cancer stem cell subpopulations. However, similar phenomenon were not observed in a cell model. The herb-ingredient-target network analysis further identified a total of 81 genes closely correlated with the breast cancer chemoprevention effects of XIAOPI. Cytokine array analysis further validated CXCL-1 as the key target of XIAOPI both in vitro and in vivo. Evaluation of the mechanism demonstrated that CXCL-1 administration significantly abrogated the metastatic inhibition effects of XIAOPI on breast cancer migration, invasion, stem cells subpopulations, epithelial-mesenchymal transition(EMT), or mammosphere formation abilities. Overall, our study provides experimental evidence and molecular mechanisms that may facilitate the safe and effective use of herbal medicine for the prevention of breast cancer growth or metastasis, and may lead to CXCL-1-based therapeutic strategies for mammary malignancies.
基金：
National Natural Science Foundation of China [81402173, 81573651, 81703764]; Guangzhou Science and Technology Program key projects [201506010098]; Guangdong Science and Technology Departmentcience and Technology Department [2016A030306025]; Guangdong Science and Technology Department [2014A020221047]; Guangdong High-level university construction project [A1-AFD018161Z1510]; Guangdong High-level Personnel of Special Support Program [A1-3002-16-111-003]; International Postdoctoral Exchange Fellowship Program; China Postdoctoral Science Foundation [2016M592585]; Guangzhou Genedenovo Biotechnology Co., Ltd
第一作者机构：[1]The Research Centre for Integrative Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.[2]Integrative Research Labrotary of Breast Cancer, Discipline of Integrated Chinese and Western Medicine, The second afliated hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China
通讯作者：
通讯机构：[1]The Research Centre for Integrative Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.[2]Integrative Research Labrotary of Breast Cancer, Discipline of Integrated Chinese and Western Medicine, The second afliated hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China[3]Post-Doctoral Research Center, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China
推荐引用方式(GB/T 7714)：
Wang Neng,Zheng Yifeng,Gu Jiangyong,et al.Network-pharmacology-based validation of TAMS/CXCL-1 as key mediator of XIAOPI formula preventing breast cancer development and metastasis[J].SCIENTIFIC REPORTS.2017,7:doi:10.1038/s41598-017-15030-3.
APA：
Wang, Neng,Zheng, Yifeng,Gu, Jiangyong,Cai, Youli,Wang, Shengqi...&Wang, Zhiyu.(2017).Network-pharmacology-based validation of TAMS/CXCL-1 as key mediator of XIAOPI formula preventing breast cancer development and metastasis.SCIENTIFIC REPORTS,7,
MLA：
Wang, Neng,et al."Network-pharmacology-based validation of TAMS/CXCL-1 as key mediator of XIAOPI formula preventing breast cancer development and metastasis".SCIENTIFIC REPORTS 7.(2017)
