机构：[1]First Affiliated Hospital of Shenzhen University, Shenzhen, China深圳市第二人民医院深圳医学信息中心[2] School of Medicine, Jinan University, Guangzhou, China[3] Department of Clinical Laboratory, School of Medicine, Shanghai Tenth People’s Hospital, Tongji University, Shanghai, China[4] Department of hospital infection control, Guangzhou Hospital of Traditional Chinese Medicine, Guangzhou, China[5] School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China[6] Guangdong Province Engineering Technology Center for Molecular Probes & Biomedical Imaging, Guangzhou, China
Two ruthenium(II) complexes, [Ru(phen)(2)HMPIP](2+) (1) and [Ru(phen)(2)MHPIP](2+) (2), have been synthesized and characterized by elemental analysis, ESI-MS, and H-1 NMR spectroscopy. The DNA-binding properties of 1 and 2 have been investigated by electronic and emission spectra and viscosity experiments. The results show that both 1 and 2 can bind to DNA in intercalating mode, with 1 exhibiting stronger binding affinity. These were confirmed by the strong hypochromism at IL and MLCT absorption bands in both complexes when DNA was added into solution, and the increase in relative viscosity of CT-DNA in the presence of both complexes. Moreover, the calculated intrinsic binding constant for 1 and 2 from the decay of electronic spectra is 3.82 x 10(5) and 2.06 x 10(5) M-1, respectively. Finally, the effects of the substituent groups on the DNA-binding behavior of ruthenium(II) complexes have also been rationally discussed by computer calculation of density functional theory (DFT) methods. [GRAPHICS]
基金：
National Nature Science Foundation of ChinaNational Natural Science Foundation of China [81572926, 81603669]; Provincial Major Scientific Research Projects in Universities of Guangdong Province [2014KZDXM053]; Science and Technology Project of Guangdong Province [2014A020212312]; Natural Science Fund of GuangdongNational Natural Science Foundation of Guangdong Province [408182136045]; Science Foundation for China Postdoctor [2016M592604]
第一作者机构：[1]First Affiliated Hospital of Shenzhen University, Shenzhen, China[2] School of Medicine, Jinan University, Guangzhou, China
共同第一作者：
通讯作者：
通讯机构：[5] School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, China[6] Guangdong Province Engineering Technology Center for Molecular Probes & Biomedical Imaging, Guangzhou, China
推荐引用方式(GB/T 7714)：
Liang Shao-Yu,Chen Yu-Xin,Zeng Yong-Chang,et al.The DNA-binding behavior and DFT calculation of ruthenium(II) complexes [Ru(phen)(2)L](ClO4)(2) (L = HMOPIP and MOHPIP)[J].JOURNAL OF COORDINATION CHEMISTRY.2017,70(24):4019-4029.doi:10.1080/00958972.2017.1411593.
APA：
Liang, Shao-Yu,Chen, Yu-Xin,Zeng, Yong-Chang,Wu, Qiong,Qiu, Fanghua...&Zheng, Wen-Jie.(2017).The DNA-binding behavior and DFT calculation of ruthenium(II) complexes [Ru(phen)(2)L](ClO4)(2) (L = HMOPIP and MOHPIP).JOURNAL OF COORDINATION CHEMISTRY,70,(24)
MLA：
Liang, Shao-Yu,et al."The DNA-binding behavior and DFT calculation of ruthenium(II) complexes [Ru(phen)(2)L](ClO4)(2) (L = HMOPIP and MOHPIP)".JOURNAL OF COORDINATION CHEMISTRY 70..24(2017):4019-4029
