机构：[1]Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangdong, Guangzhou 510515, China.[2]Department of Mechanical Engineering and Manitoba Institute of Child Health, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.[3]The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangdong, Guangzhou 510120, China广东省中医院[4]Department of Biosystem Engineering, Faculty of Engineering, Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada
Spinal cord injury leads to severe sensory ormotor damage in the human body. Efforts have beenmade to activate the nerve function by trying physical and biochemical strategies. Carbon nanotubes as conductive materials have been used to transmit electrical signals to improve cell-cell communication and cross-talk, besides providing an extracellular scaffold for neurons. We reported a thermally sensitive hydrogel using copolymerization of n-isopropylacrylamide, the oligomeric amphiphilic crosslinker of polyethylene glycol diacrylate-dodecylamine-1-(2-aminoethyl)piperazine (PEGDA-DD-AEP), and single-walled carbon nanotubes. We hypothesized that carbon nanotubes in the gel could improve neurite outgrowth and nerve regeneration. In order to overcome the aggregation issue of carbon nanotubes, the hydrophobic chains of the amphiphilic crosslinker were used to stabilize the nanotubes. The carbon nanotube-poly(n-isopropylacrylamide) (PNIPAAM) hydrogel was injectable and improved the electrical conductivity. We found that the hydrogel may have potential to promote the growth of SH-SY5Y cells, with significant neurite outgrowth while electrical stimulation was given. In a spinal cord injury model, creating a 1 mm x 1 mm x 1 mm cavity at C7, we found that the hydrogel promoted nerve tissue regeneration and reduced the formation of scar tissue. Therefore, the hydrogel may be a potential repairing biomaterial for neuron network reconstruction and spinal cord regeneration.
基金：
National Natural Science Foundation of China [31371000, 51428301]; National Natural Science Foundation of Guangdong Province [s2013010016032]; NSERCNatural Sciences and Engineering Research Council of Canada; CIHR-RPPCanadian Institutes of Health Research (CIHR); China 863 Project
第一作者机构：[1]Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, Guangdong, Guangzhou 510515, China.[2]Department of Mechanical Engineering and Manitoba Institute of Child Health, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.[3]The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangdong, Guangzhou 510120, China
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推荐引用方式(GB/T 7714)：
Sang Lili,Liu Yuqing,Hua Wenxi,et al.Thermally sensitive conductive hydrogel using amphiphilic crosslinker self-assembled carbon nanotube to enhance neurite outgrowth and promote spinal cord regeneration[J].RSC ADVANCES.2016,6(31):26341-26351.doi:10.1039/c5ra20780k.
APA：
Sang, Lili,Liu, Yuqing,Hua, Wenxi,Xu, Kaige,Wang, Guobao...&Qiu, Xiaozhong.(2016).Thermally sensitive conductive hydrogel using amphiphilic crosslinker self-assembled carbon nanotube to enhance neurite outgrowth and promote spinal cord regeneration.RSC ADVANCES,6,(31)
MLA：
Sang, Lili,et al."Thermally sensitive conductive hydrogel using amphiphilic crosslinker self-assembled carbon nanotube to enhance neurite outgrowth and promote spinal cord regeneration".RSC ADVANCES 6..31(2016):26341-26351
