To establish a method to determine the content of notoginsenoside R-1, ginsenoside Rg(1) and ginsenoside Rb-1 in Panax notoginseng tablets by ultra-performance liquid chromatography(UPLC) is the objective of this study. ACQUITY UPLC HSS T3 (2.1 mm x 50 mm, 1.8 mu m) column was used with mobile phase consisted of acetonitrile (A)-water(B) by gradient elution[ acetonitrile-water volume ratio, 0 similar to 5 min, 20:80 similar to 40:60; 5 similar to 7 min, 40: 60 similar to 20: 80; 7 similar to 10 min, 20: 80] at a flow rate of 0.3 mL.min(-1) and a column temperature of 30 degrees C, the wavelength of detector was 203nm, the injection volume was 2 mu L. Notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 showed good linearity within the range of 0.03000 similar to 0.30000, 0.12009 similar to 1.20090, 0.11997 similar to 1.19970 mu g, respectively. The average recoveries were 98.28%, 99.60% and 102.02%(n=6) with RSD of 1.46%, 1.80% and 1.88%, respectively. Three batches of Panax notoginseng tablets' contents were complied with the Ch. P. 2010. Compared with HPLC, UPLC is more convenient, efficient, accurate and excellent repeatability, the established method can be used for quality control of Panax notoginseng tablets.