Characterization and determination of antioxidant components in the leaves of Camellia chrysantha (Hu) Tuyama based on composition-activity relationship approach
机构:[1]School of Pharmaceutical Science, Guangxi Medical University, Nanning 530021, China[2]Second Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510120, China[3]Department of Traditional Chinese Medicine, Guangxi First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China
Camellia chrysantha (Hu) Tuyama (CCT), an ornamental plant possessing antioxidant activity, has been infused as tea and drank for its health benefits. The antioxidant components in CCT, however, had not been clearly characterized. To quickly identify the antioxidant constituents of CCT, a composition-activity relationship strategy based on ultra high-pressure liquid chromatography coupled with linear ion trap hybrid orbitrap mass spectrometry and orthogonal partial least-squares method has been applied. As a result, 16 variables were found to make significant contributions to the 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Six of them were identified as catechin (1), epicatechin (5), vitexin (8), isovitexin (10), quercetin-7-O-beta-D-glucopyranoside (12) and kaempferol (16). The strength of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was found to be 12 > 1 > 5 > 16 > 8 > 10 by validation test. Meanwhile, a liquid chromatography-electrospray ionization-mass spectrometry method was established for quantitative determination of six marker compounds in CCT samples from different preparations. The validation of the method, including linearity, sensitivity (limitation of detection and limitation of quantification), repeatability, precision, stability, and recoveries, was carried out and demonstrated to meet the requirements of quantitative analysis. This is the first report on the comprehensive characterization and determination of chemical constituents in CCT by ultra high-pressure liquid chromatography coupled with linear ion trap hybrid orbitrap mass spectrometry. The results indicate that the composition-activity relationship approach may be a useful method for the discovery of active constituents in natural plants and the quality control of medicinal herbs. Copyright (C) 2014, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC. All rights reserved.
基金:
National Natural
Science Foundation of China (No. 81360638), Guangxi
Natural Science Foundation (2012GXNSFBA 053108), Program
for Scientific Research in Guangxi Higher Education Institutions
(200103YB026), and Youth Science Foundation of Guangxi Medical University (02602211014).
第一作者机构:[1]School of Pharmaceutical Science, Guangxi Medical University, Nanning 530021, China
通讯作者:
通讯机构:[1]School of Pharmaceutical Science, Guangxi Medical University, Nanning 530021, China[*1]School of Pharmaceutical Science, Guangxi Medical University, Nanning 530021, China.
推荐引用方式(GB/T 7714):
Wei Jin-Bin,Li Xiong,Song Hui,et al.Characterization and determination of antioxidant components in the leaves of Camellia chrysantha (Hu) Tuyama based on composition-activity relationship approach[J].JOURNAL OF FOOD AND DRUG ANALYSIS.2015,23(1):40-48.doi:10.1016/j.jfda.2014.02.003.
APA:
Wei, Jin-Bin,Li, Xiong,Song, Hui,Liang, Yong-Hong,Pan, Yu-Zheng...&Su, Zhi-Heng.(2015).Characterization and determination of antioxidant components in the leaves of Camellia chrysantha (Hu) Tuyama based on composition-activity relationship approach.JOURNAL OF FOOD AND DRUG ANALYSIS,23,(1)
MLA:
Wei, Jin-Bin,et al."Characterization and determination of antioxidant components in the leaves of Camellia chrysantha (Hu) Tuyama based on composition-activity relationship approach".JOURNAL OF FOOD AND DRUG ANALYSIS 23..1(2015):40-48