Rapid Characterization and Identification of Flavonoids in Radix Astragali by Ultra-High-Pressure Liquid Chromatography Coupled with Linear Ion Trap-Orbitrap Mass Spectrometry
机构：[1]The Second Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, P. R. China广东省中医院
A simple and effective method was established for separation and characterization of flavonoid constituents in Radix Astragali (RA) by combination of ultra-high-pressure liquid chromatography with LTQ-Orbitrap tandem mass spectrometry (u-HPLC-LTQ-Orbitrap-MSn). For three major structural types of flavonoids, the proposed fragmentation pathways and major diagnostic fragment ions of isoflavones, pterocarpans and isoflavans were investigated to trace isoflavonoid derivatives in crude plant extracts. Based on the systematic identification strategy, 48 constituents were rapidly detected and characterized or tentatively identified, many of which were first reported in RA. The u-PHLC-LTQ-Orbitrap MSn platform was proved as an effective tool for rapid qualitative analysis of secondary metabolite productions from natural resources.
基金：
Construction Scientific Research
Projects of National TCM Clinical Research Base of State
Administration of TCM China (Grant no. JDZX2012016), Joint
Research Projects of Department of Science and Technology of
Guangdong Province and Guangdong Provincial Academy of
Chinese Medical Sciences (Grant no. 2011B032200007).
Provincial Science and Technology project of Department
of Science and Technology of Guangdong Province (Grant
no. 2013B021800235).
基金编号：Grant no. JDZX2012016
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2014]版：
大类|4 区化学
小类|4 区生化研究方法4 区分析化学
最新[2025]版：
大类|4 区化学
小类|4 区生化研究方法4 区分析化学
JCR分区：
出版当年[2013]版：
Q3CHEMISTRY, ANALYTICALQ4BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q3CHEMISTRY, ANALYTICALQ4BIOCHEMICAL RESEARCH METHODS
第一作者机构：[1]The Second Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510006, P. R. China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Jing,Xu Xiao-Jie,Xu Wen,et al.Rapid Characterization and Identification of Flavonoids in Radix Astragali by Ultra-High-Pressure Liquid Chromatography Coupled with Linear Ion Trap-Orbitrap Mass Spectrometry[J].JOURNAL OF CHROMATOGRAPHIC SCIENCE.2015,53(6):945-952.doi:10.1093/chromsci/bmu155.
APA：
Zhang, Jing,Xu, Xiao-Jie,Xu, Wen,Huang, Juan,Zhu, Da-Yuan&Qiu, Xiao-Hui.(2015).Rapid Characterization and Identification of Flavonoids in Radix Astragali by Ultra-High-Pressure Liquid Chromatography Coupled with Linear Ion Trap-Orbitrap Mass Spectrometry.JOURNAL OF CHROMATOGRAPHIC SCIENCE,53,(6)
MLA：
Zhang, Jing,et al."Rapid Characterization and Identification of Flavonoids in Radix Astragali by Ultra-High-Pressure Liquid Chromatography Coupled with Linear Ion Trap-Orbitrap Mass Spectrometry".JOURNAL OF CHROMATOGRAPHIC SCIENCE 53..6(2015):945-952
