机构：[1]Functional Experiment Centre, Guangzhou Medical University, Guangzhou, China,[2]Department of Laboratory Medicine, Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China,[3]Department of Pathogenic Biology and Immunology, institute of immunology, Guangzhou Medical University, Guangzhou, China,[4]Institute of Immunology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China,[5]Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-sen University, Guangzhou, China,[6]Department of Pharmacology, Vanderbilt University, Nashville, TN, USA,[7]Department of Infectious Diseases, Affiliated No.8 Guangzhou Peoples Hospital, Guangzhou Medical University, Guangzhou, China,[8]Clinical laboratory, Traditional Chinese Medicine Hospital of Guangdong province, Guangzhou, China广东省中医院大德路总院检验科检验科大德路总院
Systematic evaluation of the role of T cells during the Schistosoma japonicum infection has not been reported, despite the fact that T cells contribute to many infectious diseases in innate immunity. Therefore, the aim of this study was to observe the properties of T cells in the liver of C57BL/6 mice infected by S.japonicum. In this report, using immuno-fluorescent histological analysis, T cells were found around hepatic granulomatous. Moreover, the flow cytometry results revealed that the percentage of hepatic T cells increased significantly after S.japonicum infection. More interestingly, a subset of CD3(-)TCR(+) cells were found and markedly increased after infection. Furthermore, expression of activation markers (CD25 and CD69) and cytokine profiles were detected in these hepatic CD3(+)TCR(+)and CD3(-)TCR(+) cells. The significantly higher level of CD69, IL-4 and IL-17 were observed in CD3(+)TCR(+) cells after infection, suggesting that CD3(+)TCR(+) cells instead of CD3(-)TCR(+) cells might play a predominant role during the infection. Finally, our results indicated that the expression of NKG2D on CD3(+)TCR(+) cells was higher than that on CD3(-)TCR(+) cells. Collectively, T cells could play an important role in the liver of C57BL/6 mouse during japonicum infection.
基金：
Natural Science Foundation of ChinaNational Natural Science Foundation of China [30901353]; Science and Technology Planning Project of Guangzhou City [2011J22007]; College Scientific Research Project in Guangzhou City [2012C117]
第一作者机构：[1]Functional Experiment Centre, Guangzhou Medical University, Guangzhou, China,
共同第一作者：
通讯作者：
通讯机构：[3]Department of Pathogenic Biology and Immunology, institute of immunology, Guangzhou Medical University, Guangzhou, China,[8]Clinical laboratory, Traditional Chinese Medicine Hospital of Guangdong province, Guangzhou, China[*1]Department of Pathogenic Biology and Immunology, Guangzhou Medical College, 510182 Guangzhou, China[*2]Clinical laboratory, Traditional Chinese Medicine Hospital of Guangdong province, 510120 Guangzhou, China
推荐引用方式(GB/T 7714)：
Xie H.,Chen D.,Li L.,et al.Immune response of γδT cells in Schistosome japonicum-infected C57BL/6 mouse liver[J].PARASITE IMMUNOLOGY.2014,36(12):658-667.doi:10.1111/pim.12135.
APA：
Xie, H.,Chen, D.,Li, L.,Yu, X.,Wu, C....&Huang, J..(2014).Immune response of γδT cells in Schistosome japonicum-infected C57BL/6 mouse liver.PARASITE IMMUNOLOGY,36,(12)
MLA：
Xie, H.,et al."Immune response of γδT cells in Schistosome japonicum-infected C57BL/6 mouse liver".PARASITE IMMUNOLOGY 36..12(2014):658-667
