机构：[1]Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, The First Affiliated Hospital Sun Yat-Sen University, Guangzhou 510080, China中山大学附属第一医院[2]Department of Neurology, Fujian Provincial Hospital, Fujian Medical University, Fuzhou 350001, China[3]Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases,The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China中山大学附属第一医院[4]Department of Neurology,The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, China广东省中医院深圳市中医院深圳医学信息中心[5]Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa 999078, Macau
Hypertension is associated with low-grade inflammation, and Toll-like receptor 4 (TLR4) has been shown to be linked to the development and maintenance of hypertension. This study aimed to investigate the effects of scutellarin (administered by oral gavage daily for 2 weeks) on brain TLR4/nuclear factor kappa B-(NF-kappa B-) mediated inflammation and blood pressure in renovascular hypertensive (using the 2-kidney, 2-clip method) rats. Immunofluorescence and western immunoblot analyses revealed that hypertension contributed to the activation of TLR4 and NF-kappa B, accompanied by significantly enhanced expression of proinflammatory mediators, such as tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and interleukin-18 (IL-18). Furthermore, expression of the antiapoptotic protein, myeloid cell leukemia-1 (Mcl1), was decreased, and the pro-apoptotic proteins, Bax and cleavedcaspase-3 p17 were increased in combined cerebral cortical/striatal soluble lysates. Scutellarin significantly lowered blood pressure and attenuated the number of activated microglia and macrophages in brains of hypertensive rats. Furthermore, scutellarin significantly reduced the expression of TLR4, NF-kappa B p65, TNF-alpha, IL-1 beta, IL-18, Bax and cleaved-caspase-3 p17, and increased the expression of Mcl1. Overall, these results revealed that scutellarin exhibits anti-inflammatory and anti-apoptotic properties and decreases blood pressure in hypertensive rats. Therefore, scutellarin may be a potential therapeutic agent in hypertension-associated diseases.
基金：
National
Basic Research Program of China (30471917), the Doctoral
Program of Higher Education of China (20110171110058),
the Guangdong Technological grant (2011B050400031), and
the Guangdong Province Natural Science Fund (Doctoral
Initiating Project, 9451040701003684).
第一作者机构：[1]Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, The First Affiliated Hospital Sun Yat-Sen University, Guangzhou 510080, China[2]Department of Neurology, Fujian Provincial Hospital, Fujian Medical University, Fuzhou 350001, China[3]Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases,The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurology, National Key Clinical Department and Key Discipline of Neurology, The First Affiliated Hospital Sun Yat-Sen University, Guangzhou 510080, China[2]Department of Neurology, Fujian Provincial Hospital, Fujian Medical University, Fuzhou 350001, China[3]Guangdong Key Laboratory for Diagnosis and Treatment of Major Neurological Diseases,The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China[4]Department of Neurology,The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, China
推荐引用方式(GB/T 7714)：
Xingyong Chen,Xiaogeng Shi,Xu Zhang,et al.Scutellarin Attenuates Hypertension-Induced Expression of Brain Toll-Like Receptor 4/Nuclear Factor Kappa B[J].MEDIATORS OF INFLAMMATION.2013,2013:doi:10.1155/2013/432623.
APA：
Xingyong Chen,Xiaogeng Shi,Xu Zhang,Huixin Lei,Simei Long...&Ruxun Huang.(2013).Scutellarin Attenuates Hypertension-Induced Expression of Brain Toll-Like Receptor 4/Nuclear Factor Kappa B.MEDIATORS OF INFLAMMATION,2013,
MLA：
Xingyong Chen,et al."Scutellarin Attenuates Hypertension-Induced Expression of Brain Toll-Like Receptor 4/Nuclear Factor Kappa B".MEDIATORS OF INFLAMMATION 2013.(2013)
