机构：[1]Department of Neurology, Guangdong No. 2 Provincial People’s Hospital, Guangzhou 510317, China[2]Department of Physiology, Southern Medical University, Guangzhou 510515, China[3]Department of Anesthesiology, Second Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510120, China[4]The Affiliated Bayi Brain Hospital, The Military General Hospital of Beijing PLA, Beijing 100700, China
Dopamine receptor 1 (D1R) plays an essential role in regulating respiratory activity in mammals, however, little is known about how this receptor acts to modulate the basic respiratory rhythmogenesis. Here, by simultaneously recording the discharge activities of biphasic expiratory (biphasic E) neurons/inspiratory (I) neurons and the XII nerve rootlets from brainstem slices, we found that the application of D1R agonist cis-(+/-)-1-(aminomethyl)-3,4-dihydro-3-phenyl-1H-2-benzopyran-5,6-diolhydrochloride (A68930, 5 mu M), or forskolin, an intracellular cAMP-increasing agent, substantially decreased respiratory cycle and expiratory time of both types of neurons, and elevated the integral amplitude and frequency of XII nerve rootlets discharge. These changes were reversed by subsequent application of their antagonists SCH-23390 and Rp-Adenosine 3',5'-cyclic monophosphorothioate triethylammonium salt hydrate (Rp-cAMPS), respectively. Importantly, after pretreatment with Rp-cAMPS, the effects of A68930 in both types of neurons were blocked, suggestive of a cAMP-dependent action of A68930. Thus, the current study indicates that D1R may modulate basic breathing rhythmogenesis via cAMP-dependent mechanisms.
基金：
This work was supported by Grants from the
National Science Foundation of China (30570670) and the Guangdong
Natural Science Foundation (5004714) to Zhong-Hai Wu.
第一作者机构：[1]Department of Neurology, Guangdong No. 2 Provincial People’s Hospital, Guangzhou 510317, China[2]Department of Physiology, Southern Medical University, Guangzhou 510515, China
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通讯作者：
推荐引用方式(GB/T 7714)：
Yong-Gang Jiao,Guo-Cai Li,Jun-Pao Chen,et al.Dopamine Receptor 1 Modulates the Discharge Activities of Inspiratory and Biphasic Expiratory Neurons via cAMP-Dependent Pathways[J].CELLULAR AND MOLECULAR NEUROBIOLOGY.2013,33(2):155-160.doi:10.1007/s10571-012-9884-5.
APA：
Yong-Gang Jiao,Guo-Cai Li,Jun-Pao Chen,Zhong-Hai Wu&Hong-tian Zhang.(2013).Dopamine Receptor 1 Modulates the Discharge Activities of Inspiratory and Biphasic Expiratory Neurons via cAMP-Dependent Pathways.CELLULAR AND MOLECULAR NEUROBIOLOGY,33,(2)
MLA：
Yong-Gang Jiao,et al."Dopamine Receptor 1 Modulates the Discharge Activities of Inspiratory and Biphasic Expiratory Neurons via cAMP-Dependent Pathways".CELLULAR AND MOLECULAR NEUROBIOLOGY 33..2(2013):155-160
