机构：[1]Department of Urology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science & Technology, Hubei PRC.华中科技大学同济医学院附属同济医院[2]Institute of Urology, the Second Hospital of Lanzhou University, Lanzhou, PRC.[3]Wuhan General Hospital of Guangzhou Military Region, Guangzhou, PRC.[4]West China Hospital, Sichuan University, Sichuan, PRC.四川大学华西医院[5]Daping Hospital, Third Military Medical University, Chongqing, PRC.[6]The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, PRC.[7]The Second Military Medical University, Changhai Hospital, Shanghai, PRC.[8]Peking University People’s Hospital, Beijing, PRC.[9]The Third Affiliated Hospital, Sun Yat-sen University, Guangdong, PRC.中山大学附属第三医院[10]The Second Hospital of Xi’an Jiaotong University, Xi’an, PRC.[11]The Second Affiliated Hospital, Sun Yat-sen University, Guangzhou, PRC.中山大学附属第二医院[12]Xiangya Hospital of Central-south University, Changsha, PRC.[13]Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, PRC.[14]Huai’an First Hospital Affiliated to Nanjing Medical University, Jiangsu, PRC.[15]The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PRC.中山大学附属第一医院[16]The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, PRC.广东省中医院[17]The First Affiliated Hospital of Southern Medical University (Nanfang Hospital), Guangzhou, PRC.[18]Affiliated Hospital of Nantong University, Jiangsu, PRC.[19]Foshan Hospital of Traditional Chinese Medicine, Guangdong, PRC.[20]Guangzhou First Municipal People’s Hospital, Guangzhou, PRC.[21]General Hospital of Guangzhou Military Command of PLA, Guangzhou, PRC.[22]The First Affiliated Hospital of Nanjing Medical University, Jiangsu, PRC.江苏省人民医院[23]The Second Affiliated Hospital of Kunming Medical College, Yunnan, PRC.[24]School of Chinese Medicine, The University of Hong Kong, Hong Kong SAR.[25]UNIMED Medical Institute, Hong Kong SAR.
Background: Catheter-associated urinary tract infection (CAUTI) is a common nosocomial device-associated infection. It is now recognized that the high infection rates were caused by the formation of biofilm on the surface of the catheters that decreases the susceptibility to antibiotics and results in anti-microbial resistance. In this study, we performed an in vitro test to explore the mechanism of biofilm formation and subsequently conducted a multi-center clinical trial to investigate the efficacy of CAUTI prevention with the application of JUC, a nanotechnology antimicrobial spray. Methods: Siliconized latex urinary catheters were cut into fragments and sterilized by autoclaving. The sterilized sample fragments were randomly divided into the therapy and control group, whereby they were sprayed with JUC and distilled water respectively and dried before use. The experimental standard strains of Escherichia coli (E. coli) were isolated from the urine samples of patients. At 16 hours and 7 days of incubation, the samples were extracted for confocal laser scanning microscopy. A total of 1,150 patients were accrued in the clinical study. Patients were randomized according to the order of surgical treatment. The odd array of patients was assigned as the therapy group (JUC), and the even array of patients was assigned as the control group (normal saline). Results: After 16 hours of culture, bacterial biofilm formed on the surface of sample fragments from the control group. In the therapy group, no bacterial biofilm formation was observed on the sample fragments. No significant increase in bacterial colony count was observed in the therapy group after 7 days of incubation. On the 7th day of catheterization, urine samples were collected for bacterial culture before extubation. Significant difference was observed in the incidence of bacteriuria between the therapy group and control group (4.52% vs. 13.04%, p < 0.001). Conclusions: In this study, the effectiveness of JUC in preventing CAUTI in a hospital setting was demonstrated in both in vitro and clinical studies.
第一作者机构：[1]Department of Urology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science & Technology, Hubei PRC.
通讯作者：
通讯机构：[24]School of Chinese Medicine, The University of Hong Kong, Hong Kong SAR.[25]UNIMED Medical Institute, Hong Kong SAR.
推荐引用方式(GB/T 7714)：
Wei He,Dongmin Wang,Zhangqun Ye,et al.Application of a nanotechnology antimicrobial spray to prevent lower urinary tract infection: a multicenter urology trial[J].JOURNAL OF TRANSLATIONAL MEDICINE.2012,10:doi:10.1186/1479-5876-10-S1-S14.
APA：
Wei He,Dongmin Wang,Zhangqun Ye,Weihong Qian,Yan Tao...&Zhiping Wang.(2012).Application of a nanotechnology antimicrobial spray to prevent lower urinary tract infection: a multicenter urology trial.JOURNAL OF TRANSLATIONAL MEDICINE,10,
MLA：
Wei He,et al."Application of a nanotechnology antimicrobial spray to prevent lower urinary tract infection: a multicenter urology trial".JOURNAL OF TRANSLATIONAL MEDICINE 10.(2012)
医学