机构：[1]Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China广东省中医院[2]The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou 510120, China广东省中医院[3]College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
Frontal affinity chromatography combined on-line with mass spectrometry (FAC-MS) is an efficient method for screening traditional Chinese medicine (TCM). This paper reports the application of FAC-MS to screening PAI-1 inhibitor from TCM. Novel small molecule inhibitors derived from natural products are particularly desirable as inhibitors of PAI-1. Several promising inhibitors of PAI-1 from TCM were identified by FAC-MS. These inhibitors included chrysophanol, rhein, corilagin, and geraniin, with 50% inhibitory concentrations (IC50) of 1.06, 37.32, 30.38, and 10.48 mu M, respectively. The results indicate that FAC-MS is a direct and fast method for screening active compounds from complex molecular systems. The sample in this method is a mixture, so it is suitable for the screening Chinese herb extracts.
基金：
Major Program of Science and Technique Project of Guangdong Province [2009A030100006]; Research Program of Guangdong Provincial Hospital of Chinese Medicine [2007E199, 092YTS16]
第一作者机构：[1]Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China[2]The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou 510120, China
通讯作者：
通讯机构：[1]Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou 510006, China[2]The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou 510120, China[3]College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
推荐引用方式(GB/T 7714)：
Yuan Xiaohong,Hu Daoju,Zhu Wei,et al.Application of frontal affinity chromatography combined on-line with mass spectrometry to screening PAI-1 inhibitors from traditional Chinese medicine[J].ANALYTICAL METHODS.2012,4(11):3744-3747.doi:10.1039/c2ay25696g.
APA：
Yuan, Xiaohong,Hu, Daoju,Zhu, Wei,Xu, Xiaojie&Lu, Chuanjian.(2012).Application of frontal affinity chromatography combined on-line with mass spectrometry to screening PAI-1 inhibitors from traditional Chinese medicine.ANALYTICAL METHODS,4,(11)
MLA：
Yuan, Xiaohong,et al."Application of frontal affinity chromatography combined on-line with mass spectrometry to screening PAI-1 inhibitors from traditional Chinese medicine".ANALYTICAL METHODS 4..11(2012):3744-3747
