机构：[1]MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, Guangzhou 510631, Guangdong Province, China[2]Department of Anesthesiology, the Second Affiliated Clinical Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510120, Guangdong Province, China[3]Key Laboratory of Optocleclronic Science and Technology for Medicine of Ministry of Education of China, Fujian Normal University, Fuzhou 350007, Fujian, China[4]Department of Surgery, the First Affiliated Hospital, Sun YatSen University, Guangzhou 510080, Guangdong Province, China中山大学附属第一医院[5]Laser Medicine Lab, Department of Biomedical Engineering, School of PreClinical Medicine of Sun YetSen University, Guangzhou Guangdong 510080, China
The main objective of the present work is to study the influence of heat treatment on the esophageal cancer detection using the diffuse reflectance (DR) spectral intensity ratio R540/R575 of oxygenated hemoglobin (HbO(2)) absorption bands to distinguish the epithelial tissues of normal human esophagus and moderately differentiated esophageal squamous cell carcinoma (ESCC) at different heat treatment temperature of 20, 37, 42, 50, and 60A degrees C, respectively. The DR spectra for the epithelial tissues of the normal esophagus and ESCC in vitro at different heat-treatment temperature in the wavelength range 400-650 nm were measured with a commercial optical fiber spectrometer. The results indicate that the average DR spectral intensity overall enhancement with concomitant increase of heat-treatment temperature for the epithelial tissues of normal esophagus and ESCC, but the average DR spectral intensity for the normal esophageal epithelial tissues is relatively higher than that for ESCC epithelial tissues at the same heat-treatment temperature. The mean R540/R575 ratios of ESCC epithelial tissues were always lower than that of normal esophageal epithelial tissues at the same temperature, and the mean R540/R575 ratios of the epithelial tissues of the normal esophagus and ESCC were decreasing with the increase of different heat-treatment temperatures. The differences in the mean R540/R575 ratios between the epithelial tissues of normal esophagus and ESCC were 13.33, 13.59, 11.76, and 11.11% at different heat-treatment temperature of 20, 37, 42, and 50A degrees C, respectively. These results also indicate that the DR intensity ratio R540/R575 of the hemoglobin bands is a useful tool for discrimination between the epithelial tissues of normal esophagus and ESCC in the temperature range from room temperature to 50A degrees C, but it was non-effective at 60A degrees C or over 60A degrees C.
基金：
National Natural Science Foundation of China
(60778047), the Natural Science Foundation of
Guangdong Province (grant no. 9251063101000009),
the Key Science and Technology Project of Guang
dong Province of China (2005B50101015 and
2008B090500125), the Key Science and Technology
Project of Guangzhou of China (2008Z1D391), as
well as Specialized Research Fund for the Doctoral
Program of Higher Education (200805740003).
第一作者机构：[1]MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, Guangzhou 510631, Guangdong Province, China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhao Q. L.,Guo Z. Y.,Si J. L.,et al.Heat treatment of human esophageal tissues: Effect on esophageal cancer detection using oxygenated hemoglobin diffuse reflectance ratio[J].LASER PHYSICS.2011,21(3):559-565.doi:10.1134/S1054660X11050380.
APA：
Zhao, Q. L.,Guo, Z. Y.,Si, J. L.,Wei, H. J.,Yang, H. Q....&Li, X. Y..(2011).Heat treatment of human esophageal tissues: Effect on esophageal cancer detection using oxygenated hemoglobin diffuse reflectance ratio.LASER PHYSICS,21,(3)
MLA：
Zhao, Q. L.,et al."Heat treatment of human esophageal tissues: Effect on esophageal cancer detection using oxygenated hemoglobin diffuse reflectance ratio".LASER PHYSICS 21..3(2011):559-565
