机构：[1]Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, Hunan Normal University, Changsha 410081 Hunan, People’s Republic of China[2]Key Laboratory of Heredity & Healthy Birth of Hunan Province, Changsha 410007 Hunan, People’s Republic of China[3]Department of Pharmacology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430022, People’s Republic of China[4]Guangdong Provincial Hospital of TCM, Guangzhou 510120, People’s Republic of China广东省中医院[5]College of Life Sciences, Hunan Normal University, Changsha 410081 Hunan, People’s Republic of China
The objective of this study was to investigate the influences of carbonyl stress induced by malondialdehyde (MDA), a typical intermediate of lipid peroxidation, on intracellular free Ca2+ concentration ([Ca2+](i)) alterations in cultured hippocampal neurons of rat. The microphotographic study clearly demonstrated that the hippocampal neurons became gradually damaged following exposure to different concentrations of MDA. Further study indicated that the plasma membrane Ca2+-ATPase (PMCA) activity was inhibited by MDA in a concentration- and time-dependent manner. The supplementation of 100 mu M MDA was found to cause a notable early phase increase of [Ca2+](i) in hippocampal neuron cultures followed by a more pronounced late-phase elevation of [Ca2+](i). Such effect of MDA was prevented by the addition of nimodipine, an inhibitor of L-type calcium channel or by an extracellular Ca2+ chelator EGTA. The identification of the calcium signalling pathways were studied by applying U73122, an inhibitor of PL-C, and H-89, an inhibitor of protein kinase A (PKA), showing the involvement of PL-C/IP3 pathway but not the PKA/cAMP pathway. These results suggested that MDA-related carbonyl stress caused damages of rat hippocampal neurons by triggering Ca2+ influx and influencing Ca2+ homeostasis in cultured neurons, and also MDA may act as a signalling molecule regulating Ca2+ release from intracellular stores.
基金：
Scientific Bureau of Hunan Province [06FJ3001]; National Key Basic Research Programme-the 973-Projects National Basic Research Program of China [2007CB507404]
第一作者机构：[1]Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, Hunan Normal University, Changsha 410081 Hunan, People’s Republic of China[2]Key Laboratory of Heredity & Healthy Birth of Hunan Province, Changsha 410007 Hunan, People’s Republic of China[5]College of Life Sciences, Hunan Normal University, Changsha 410081 Hunan, People’s Republic of China
通讯作者：
通讯机构：[1]Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education, Hunan Normal University, Changsha 410081 Hunan, People’s Republic of China[2]Key Laboratory of Heredity & Healthy Birth of Hunan Province, Changsha 410007 Hunan, People’s Republic of China[5]College of Life Sciences, Hunan Normal University, Changsha 410081 Hunan, People’s Republic of China
推荐引用方式(GB/T 7714)：
Cai Jianguang,Chen Jianguo,He Hong,et al.Carbonyl stress: malondialdehyde induces damage on rat hippocampal neurons by disturbance of Ca2+ homeostasis[J].CELL BIOLOGY AND TOXICOLOGY.2009,25(5):435-445.doi:10.1007/s10565-008-9097-3.
APA：
Cai, Jianguang,Chen, Jianguo,He, Hong,Yin, Zhaochu,Zhu, Zerui&Yin, Dazhong.(2009).Carbonyl stress: malondialdehyde induces damage on rat hippocampal neurons by disturbance of Ca2+ homeostasis.CELL BIOLOGY AND TOXICOLOGY,25,(5)
MLA：
Cai, Jianguang,et al."Carbonyl stress: malondialdehyde induces damage on rat hippocampal neurons by disturbance of Ca2+ homeostasis".CELL BIOLOGY AND TOXICOLOGY 25..5(2009):435-445
