This article has been corrected: Due to errors during figure assembly, the Figure 8A control image is incorrect. The corrected Figure 8 is shown below. In addition, in the sections RESULTS and MATERIALS AND METHODS, the terms “nude mice” or “athymic nude mice” are incorrect; they have been changed in the paragraphs below to 'NOD.Cg-PrkdcscidIl2rgtm1Sug/ JicCrl (NOG) mice'. The authors declare that these corrections do not change the results or conclusions of this paper. Original article: Oncotarget. 2015; 6:35813-35829. https://doi.org/10.18632/oncotarget.5358 In vivo xenograft studies in the NOD.Cg-PrkdcscidIl2rgtm1Sug/JicCrl (NOG) mice For the xenograft assay, female NOD.Cg-PrkdcscidIl2rgtm1Sug/JicCrl (NOG) mice (about 8 weeks of age) were anesthetized with sodium pentobarbital (50 mg/kg) in a sterile environment. Then, OVCAR3, OVCAR3/CTHRC1-siRNA, SKOV3 and SKOV3/pcDNA3.1-CTHRC1 cells (2 × 106) in 50 μl of PBS were subcutaneously injected into the flank of individual NOD.Cg-PrkdcscidIl2rgtm1Sug/JicCrl (NOG) mice using one-milliliter syringes with hypodermic needles. Different time after inoculation, the mice were killed and tumor tissues were immersed in 10% neutral buffered formalin overnight for the immunohistochemical study. For IHC staining, the primary antibodies were E-cadherin, vimentin, Snail, Slug and Twist (Abcam, USA). Enzyme-linked immunosorbent assay was used to measure the secreted CTHRC1 protein level in the serum. Tumor growth was monitored by a caliper and an IVIS Imaging System (Xenogen). Living Image and Xenogen software was used to analyze the images and bioluminescent signals. We analyzed H&E staining status and BLI images of mice to define tumor metastasis. All animal experiments were performed according to the protocols approved by the medical ethical committee of Sun Yat-sen University. CTHRC1 is closely associated with EMT status in xenograft models To reveal the role of CTHRC1 in the metastasis and EMT of EOC cells, we explored the metastatic activity of OVCAR3/ CTHRC1-siRNA and SKOV3/pcDNA3.1-CTHRC1 in NOD.Cg-PrkdcscidIl2rgtm1Sug/JicCrl (NOG) mice. We found that OVCAR3/CTHRC1-siRNA cells predominantly localized to tumor nodules in the primary injection sites compared to control. However, SKOV3/pcDNA3.1-CTHRC1 cells formed multiple tumors in the peritoneum cavity. The number of metastatic nodules was measured according to the fluorescence signal and H&E staining. As shown in Figure 8A and 8B, SKOV3/ pcDNA3.1-CTHRC1 cells formed a greater number of metastases than SKOV3 cells in abdomen (3.00 ± 1.05 vs. 1.30 ± 0.48, p < 0.01, respectively). These in vivo results confirmed the role of CTHRC1 in the promotion of EOC invasion. © Hou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.