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Extraction, purification and characterization of sulphated polysaccharide from Bellamya quadrata and its stabilization roles on atherosclerotic plaque.

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资源类型：
Pubmed体系：
作者：
Xiong Qingping[1,2,4,*1] * ; Hu Youdong[3] * ; Ye Xianying[4] * ; Song Zhuoyue[4] ; Yuan Jun[1] ; Xiong Boyang[6] ; Jing Yi[1,2] ; Shi Yingying[1,2] ; Xu Tingting[1,2] ; Wu Jun[1] ; Zhang Qianghua[1,2] ; Liang Jian[1,4,*1,*2] ; Zhou Li[1,5,*1,*3] ;
机构： [1]Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huai'an 223003, Jiangsu, China [2]National & Local Joint Engineering Research Center for Mineral Salt Deep Utilization, Huaiyin Institute of Technology, Huai'an 223003, China [3]Department of Geriatric Medicine, Huai'an Second People's Hospital, Huai'an 223002, Jiangsu, China [4]Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, China [5]Department of Intensive Care Unit, Dazhou Central Hospital, Dazhou 635000, Sichuan, China [6]Department of Pharmacy, Jiangsu Food & Pharmaceutical Science College, Huai'an 223003, Jiangsu, China
出处：
DOI： 10.1016/j.ijbiomac.2020.02.243
ISSN：

关键词： Sulphated polysaccharide from Bellamya quadrata Preparation and characterization Stabilization role on atherosclerotic plaque

摘要：
The aim of this paper was to investigate the extraction, purification and characterization of sulphated polysaccharide (BQPS) from Bellamya quadrata and its stabilization roles on atherosclerotic plaque. Firstly, crude polysaccharide (CBQP) from Bellamya quadrata was extracted by protease enzyme assisted extraction. Moreover, its optimal parameters were obtained by the response surface method as follows: the ratio of water to raw material of 24:1, enzyme dosage of 285 U/g, enzymolysis pH value of 4.7 and temperature of 67 °C. Secondly, CBQP was further purified to obtain the target polysaccharide BQPS by Q Sepharose Fast Flow and Sephacryl S-400 gel column chromatography. Then, the characterization of BQPS revealed that it possessed a total polysaccharide content of 91.88 ± 1.23%, sulfuric acid group content of 9.12 ± 1.59% and molecular weight of 91.1 kDa. BQPS was only consisted of glucose without any proteins. Finally, BQPS was confirmed to have a significant stabilizing effect on atherosclerotic plaque and its mechanism was related to the selective promotion of autophagy with the precisely right strength. Copyright © 2020 Elsevier B.V. All rights reserved.

基金：
the National Natural Science Foundation of China (No. 81503387), Six Talent Peaks Project in Jiangsu Province (2017-YY-003), Natural Science Fund for Colleges and Universities in Jiangsu Province of China (No.19KJA430015) and Open Funds of Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research (LPRK201903).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类 | 2 区 化学
小类 | 2 区 应用化学 2 区 高分子科学 3 区 生化与分子生物学
最新[2025]版：
大类 | 2 区 生物学
小类 | 2 区 生化与分子生物学 2 区 应用化学 2 区 高分子科学
第一作者：
第一作者机构： [1]Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huai'an 223003, Jiangsu, China [2]National & Local Joint Engineering Research Center for Mineral Salt Deep Utilization, Huaiyin Institute of Technology, Huai'an 223003, China [4]Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, China [*1]Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huai'an 223003, Jiangsu, China.
共同第一作者：
通讯作者：
通讯机构： [1]Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huai'an 223003, Jiangsu, China [2]National & Local Joint Engineering Research Center for Mineral Salt Deep Utilization, Huaiyin Institute of Technology, Huai'an 223003, China [4]Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, China [5]Department of Intensive Care Unit, Dazhou Central Hospital, Dazhou 635000, Sichuan, China [*1]Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huai'an 223003, Jiangsu, China. [*2]Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China [*3]Department of Intensive Care Unit, Dazhou Central Hospital, Dazhou 635000, Sichuan, China
推荐引用方式(GB/T 7714)：
Xiong Qingping,Hu Youdong,Ye Xianying,et al.Extraction, purification and characterization of sulphated polysaccharide from Bellamya quadrata and its stabilization roles on atherosclerotic plaque.[J].International journal of biological macromolecules.2020,152:314-326.doi:10.1016/j.ijbiomac.2020.02.243.
APA：
Xiong Qingping,Hu Youdong,Ye Xianying,Song Zhuoyue,Yuan Jun...&Zhou Li.(2020).Extraction, purification and characterization of sulphated polysaccharide from Bellamya quadrata and its stabilization roles on atherosclerotic plaque..International journal of biological macromolecules,152,
MLA：
Xiong Qingping,et al."Extraction, purification and characterization of sulphated polysaccharide from Bellamya quadrata and its stabilization roles on atherosclerotic plaque.".International journal of biological macromolecules 152.(2020):314-326

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