机构：[1]Department of General Surgery, Guangdong Second Provincial General Hospital, Guangzhou, China[2]The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China[3]Department of Gastrointestinal Surgery, Meizhou People’s Hospital, Meizhou, China[4]Shunde Hospital of Guangzhou University of Traditional Chinese Medicine, Foshan, China深圳市中医院深圳医学信息中心[5]Department of Laboratory Medicine, Guangdong Second Provincial General Hospital, Guangzhou, China
Objective: Titanium oxide (TiO2) acts as a photosensitizer in photodynamic therapy by mediating
reactive oxygen species (ROS)-induced endoplasmic reticulum (ER) stress. This study aimed
to investigate the effect of TiO2 on ER stress in liver cancer cells.
Methods: Normal human liver and human hepatocarcinoma cell lines were incubated with
various concentrations of TiO2 nanotubes for 48 hours. Cell growth, apoptosis, cell cycle, and
cellular ROS were detected. Expression levels of ER stress sensors (PERK and ATF6) and Bax
were evaluated by western blot. The effect of TiO2 on liver cancer growth was also investigated
in mice in vivo.
Results: TiO2 inhibited cell growth, increased apoptosis and cellular ROS levels, and arrested the
cell cycle in G1 stage in liver cancer cells. TiO2 also increased PERK, ATF6, and Bax expression
levels in liver cancer cells in dose-dependent manners. TiO2 had no significant effect on cell
growth, apoptosis, ROS level, cell cycle distribution, or PERK, ATF6, or Bax expression in normal
liver cells. TiO2 administration reduced tumor volume and increased PERK, Bax, and ATF6
expression levels in tumor tissues in vivo.
Conclusions: TiO2 nanoparticles increased ROS-induced ER stress and activated the PERK/
ATF6/Bax axis in liver cancer cells in vitro and in vivo
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区药学
最新[2025]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区药学
第一作者：
第一作者机构：[1]Department of General Surgery, Guangdong Second Provincial General Hospital, Guangzhou, China[2]The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China[3]Department of Gastrointestinal Surgery, Meizhou People’s Hospital, Meizhou, China
通讯作者：
通讯机构：[1]Department of General Surgery, Guangdong Second Provincial General Hospital, Guangzhou, China[2]The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China[*1]Department of General Surgery, Guangdong Second Provincial General Hospital, The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510317, P.R. China
推荐引用方式(GB/T 7714)：
Li Zhiwang,He Jingliang,Li Bowei,et al.Titanium dioxide nanoparticles induce endoplasmic reticulum stress-mediated apoptotic cell death in liver cancer cells.[J].The Journal of international medical research.2020,48(4):300060520903652.doi:10.1177/0300060520903652.
APA：
Li Zhiwang,He Jingliang,Li Bowei,Zhang Jinqian,He Ke...&Xiang Guoan.(2020).Titanium dioxide nanoparticles induce endoplasmic reticulum stress-mediated apoptotic cell death in liver cancer cells..The Journal of international medical research,48,(4)
MLA：
Li Zhiwang,et al."Titanium dioxide nanoparticles induce endoplasmic reticulum stress-mediated apoptotic cell death in liver cancer cells.".The Journal of international medical research 48..4(2020):300060520903652
