机构：[1]Department of Encephalopathy, Anyang Hospital of Traditional Chinese Medicine, Anyang, China.[2]Out-Patient Department, Fourth Medical Center of the Chinese PLA General Hospital, Beijing, China.[3]Laboratory of Shock and Multiple Organ Dysfunction, Trauma Research Center, Fourth Medical Center of the Chinese PLA General Hospital, Beijing, China.[4]Research Center of Trauma Repair and Tissue Regeneration, Medical Innovation Research Department, Chinese PLA General Hospital, Beijing, China.[5]Chinese People's Armed Police Force Academy, Langfang, China.[6]Chengdu Hospital of Sichuan Provincial Corps, Chinese People's Armed Police Force, Chengdu, China.[7]Department of Burns and Wound Repair Surgery, Guangdong General Hospital and Guangdong Academy of Medical Sciences, Guangzhou, China.广东省人民医院[8]Department of Acupuncture and Moxibustion, Chinese PLA General Hospital, Beijing, China.
To investigate whether the mechanism underlying the anti-inflammatory effects of electroacupuncture (EA) at ST36 involves dopamine (DA) and its receptor and whether it is mediated by the vagus nerve in a rat model of intestinal ischaemia-reperfusion (I/R) injury.
Rats were subjected to gut ischaemia for 30 min and then received EA for 30 min with or without abdominal vagotomy or intraperitoneal administration of butaclamol (D1 receptor antagonist) or spiperone (D2 receptor antagonist). Plasma levels of DA and tumour necrosis factor (TNF)-α were assessed 1 or 4 h after reperfusion. Myeloperoxidase (MPO) activity and malondialdehyde (MDA) content in intestinal tissues were assessed using enzyme-linked immunosorbent assay (ELISA) kits. Intestinal tissue injury was assessed by observation of the pathological lesions and permeability to 4 kDa fluorescein isothiocyanate (FITC)-dextran.
EA significantly increased levels of DA and lowered levels of TNF-α. EA also inhibited intestinal levels of MPO and MDA and intestinal tissue injury and decreased intestinal permeability to FITC-dextran. Abdominal vagotomy and intraperitoneal administration of butaclamol (but not spiperone) inhibited the effects of EA.
These findings suggest that EA at ST36 could attenuate intestinal I/R-induced inflammatory injury and that the underlying mechanism may involve EA-induced increases in levels of DA, mediated by the vagus nerve and D1 receptors.
基金：
This study was supported by the National Natural
Science Foundation of China (grant no. 81774422).
第一作者机构：[1]Department of Encephalopathy, Anyang Hospital of Traditional Chinese Medicine, Anyang, China.
通讯作者：
通讯机构：[3]Laboratory of Shock and Multiple Organ Dysfunction, Trauma Research Center, Fourth Medical Center of the Chinese PLA General Hospital, Beijing, China.[4]Research Center of Trauma Repair and Tissue Regeneration, Medical Innovation Research Department, Chinese PLA General Hospital, Beijing, China.[*1]Laboratory of Shock and Multiple Organ Dysfunction, Trauma Research Center, Fourth Medical Center of the Chinese PLA General Hospital, Research Center of Trauma Repair and Tissue Regeneration, Medical Innovation Research Department of Chinese PLA General Hospital, No. 51 Fu Cheng Road, Beijing 100048, China.
推荐引用方式(GB/T 7714)：
Li Yumeng,Xu Guochen,Hu Sen,et al.Electroacupuncture alleviates intestinal inflammation and barrier dysfunction by activating dopamine in a rat model of intestinal ischaemia.[J].ACUPUNCTURE IN MEDICINE.2021,39(3):208-216.doi:10.1177/0964528420922232.
APA：
Li Yumeng,Xu Guochen,Hu Sen,Wu Hong,Dai Yuelong...&Shi Xian.(2021).Electroacupuncture alleviates intestinal inflammation and barrier dysfunction by activating dopamine in a rat model of intestinal ischaemia..ACUPUNCTURE IN MEDICINE,39,(3)
MLA：
Li Yumeng,et al."Electroacupuncture alleviates intestinal inflammation and barrier dysfunction by activating dopamine in a rat model of intestinal ischaemia.".ACUPUNCTURE IN MEDICINE 39..3(2021):208-216
